Literature DB >> 1849255

A promoter trap in embryonic stem (ES) cells selects for integration of DNA into CpG islands.

D Macleod1, R Lovell-Badge, S Jones, I Jackson.   

Abstract

An analysis of several G418-resistant ES cell lines produced by electroporation of a promoterless neo gene (NASTI), shows an enrichment for integrations within, or adjacent to, CpG islands. A detailed analysis of two of the cell lines reveals short regions of homology between the genomic target DNA and the construct ends, and that recombination may be mediated by DNA Topoisomerase I. The DNA flanking the insert detects transcription of endogenous genes, and in one cell line divergent transcripts are detected. This use of ES cells should provide an effective and efficient means of creating insertional mutations in mice.

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Year:  1991        PMID： 1849255      PMCID： PMC333529          DOI： 10.1093/nar/19.1.17

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  42 in total

1.  Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells.

Authors:  K R Thomas; M R Capecchi
Journal:  Cell       Date:  1987-11-06       Impact factor: 41.582

Review 2.  CpG-rich islands and the function of DNA methylation.

Authors:  A P Bird
Journal:  Nature       Date:  1986 May 15-21       Impact factor: 49.962

3.  Association of crossover points with topoisomerase I cleavage sites: a model for nonhomologous recombination.

Authors:  P Bullock; J J Champoux; M Botchan
Journal:  Science       Date:  1985-11-22       Impact factor: 47.728

4.  Nonhomologous recombination in mammalian cells: role for short sequence homologies in the joining reaction.

Authors:  D B Roth; J H Wilson
Journal:  Mol Cell Biol       Date:  1986-12       Impact factor: 4.272

5.  Establishment in culture of pluripotential cells from mouse embryos.

Authors:  M J Evans; M H Kaufman
Journal:  Nature       Date:  1981-07-09       Impact factor: 49.962

6.  Multiple transcription start sites, DNase I-hypersensitive sites, and an opposite-strand exon in the 5' region of the CHO dhfr gene.

Authors:  P J Mitchell; A M Carothers; J H Han; J D Harding; E Kas; L Venolia; L A Chasin
Journal:  Mol Cell Biol       Date:  1986-02       Impact factor: 4.272

7.  Selection for precise chromosomal targeting of a dominant marker by homologous recombination.

Authors:  J R Dorin; J D Inglis; D J Porteous
Journal:  Science       Date:  1989-03-10       Impact factor: 47.728

8.  Human glyceraldehyde-3-phosphate dehydrogenase: mRNA levels and enzyme activity in developing muscle.

Authors:  Y H Edwards; J C Lloyd; S L McMillan; F J Benham
Journal:  Mol Cell Biol       Date:  1985-08       Impact factor: 4.272

9.  Compilation of DNA strand exchange sites for non-homologous recombination in somatic cells.

Authors:  A K Konopka
Journal:  Nucleic Acids Res       Date:  1988-03-11       Impact factor: 16.971

10.  The genes for the alpha 1(IV) and alpha 2(IV) chains of human basement membrane collagen type IV are arranged head-to-head and separated by a bidirectional promoter of unique structure.

Authors:  E Pöschl; R Pollner; K Kühn
Journal:  EMBO J       Date:  1988-09       Impact factor: 11.598
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  10 in total

1.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1991-04-11       Impact factor: 16.971

2.  A molecular strategy designed for the rapid screening of gene traps based on sequence identity and gene expression pattern in adult mice.

Authors:  D Holzschu; L Lapierre; D Neubaum; W H Mark
Journal:  Transgenic Res       Date:  1997-01       Impact factor: 2.788

3.  Processing of DNA prior to illegitimate recombination in mouse cells.

Authors:  G Henderson; J P Simons
Journal:  Mol Cell Biol       Date:  1997-07       Impact factor: 4.272

4.  The effects of terminal heterologies on gene targeting by insertion vectors in embryonic stem cells.

Authors:  S Kumar; J P Simons
Journal:  Nucleic Acids Res       Date:  1993-04-11       Impact factor: 16.971

5.  A series of vectors that simplify mammalian gene targeting.

Authors:  A J Brookes; B J Stevenson; D J Porteous; J R Dorin
Journal:  Transgenic Res       Date:  1993-07       Impact factor: 2.788

Review 6.  Epigenetic control of recombination in the immune system.

Authors:  Yehudit Bergman; Howard Cedar
Journal:  Semin Immunol       Date:  2010-09-15       Impact factor: 11.130

7.  A model for the mechanism of precise integration of a microinjected transgene.

Authors:  M McFarlane; J B Wilson
Journal:  Transgenic Res       Date:  1996-05       Impact factor: 2.788

8.  Mapping candidate hotspots of meiotic recombination in segments of human DNA cloned in the yeast Saccharomyces cerevisiae.

Authors:  M Mucha; J Król; A Goc; J Filipski
Journal:  Mol Genet Genomics       Date:  2003-08-26       Impact factor: 3.291

Review 9.  Insertional mutagenesis in transgenic mice.

Authors:  T Rijkers; A Peetz; U Rüther
Journal:  Transgenic Res       Date:  1994-07       Impact factor: 2.788

10.  Fluorescence-activated sorting of totipotent embryonic stem cells expressing developmentally regulated lacZ fusion genes.

Authors:  S Reddy; H Rayburn; H von Melchner; H E Ruley
Journal:  Proc Natl Acad Sci U S A       Date:  1992-08-01       Impact factor: 11.205
  10 in total

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