OBJECTIVES: Infliximab is the first anti-TNFalpha accepted by the Food and Drug Administration for use in inflammatory bowel disease treatment. Few clinical, biological and genetic factors tend to predict response in Crohn's disease (CD) patient subcategories, none widely predicting response to infliximab. DESIGN AND METHODS: Twenty CD patients showing clinical response or non response to infliximab were used for serum proteomic profiling on Surface Enhanced Lazer Desorption Ionisation-Time of Flight-Mass Spectrometry (SELDI-TOF-MS), each before and after treatment. Univariate and multivariate data analysis were performed for prediction and characterization of response to infliximab. RESULTS: We obtained a model of classification predicting response to treatment and selected relevant potential biomarkers, among which platelet aggregation factor 4 (PF4). We quantified PF4, sCD40L and IL-6 by ELISA for correlation studies. CONCLUSIONS: This first proteomic pilot study on response to infliximab in CD suggests association between platelet metabolism and response to infliximab and requires validation studies on a larger cohort of patients.
OBJECTIVES: Infliximab is the first anti-TNFalpha accepted by the Food and Drug Administration for use in inflammatory bowel disease treatment. Few clinical, biological and genetic factors tend to predict response in Crohn's disease (CD) patient subcategories, none widely predicting response to infliximab. DESIGN AND METHODS: Twenty CDpatients showing clinical response or non response to infliximab were used for serum proteomic profiling on Surface Enhanced Lazer Desorption Ionisation-Time of Flight-Mass Spectrometry (SELDI-TOF-MS), each before and after treatment. Univariate and multivariate data analysis were performed for prediction and characterization of response to infliximab. RESULTS: We obtained a model of classification predicting response to treatment and selected relevant potential biomarkers, among which platelet aggregation factor 4 (PF4). We quantified PF4, sCD40L and IL-6 by ELISA for correlation studies. CONCLUSIONS: This first proteomic pilot study on response to infliximab in CD suggests association between platelet metabolism and response to infliximab and requires validation studies on a larger cohort of patients.
Authors: Wanshu Ma; Hyea Jin Gil; Noelia Escobedo; Alberto Benito-Martín; Pilar Ximénez-Embún; Javier Muñoz; Héctor Peinado; Stanley G Rockson; Guillermo Oliver Journal: JCI Insight Date: 2020-07-09
Authors: Anna Vaiopoulou; Maria Gazouli; Aggeliki Papadopoulou; Athanassios K Anagnostopoulos; George Karamanolis; George E Theodoropoulos; Amosy M'Koma; George T Tsangaris Journal: J Pediatr Gastroenterol Nutr Date: 2015-01 Impact factor: 2.839
Authors: C Trocmé; H Marotte; A Baillet; B Pallot-Prades; J Garin; L Grange; P Miossec; J Tebib; F Berger; M J Nissen; R Juvin; F Morel; P Gaudin Journal: Ann Rheum Dis Date: 2008-07-29 Impact factor: 19.103
Authors: Douglas K Brubaker; Manu P Kumar; Evan L Chiswick; Cecil Gregg; Alina Starchenko; Paige N Vega; Austin N Southard-Smith; Alan J Simmons; Elizabeth A Scoville; Lori A Coburn; Keith T Wilson; Ken S Lau; Douglas A Lauffenburger Journal: Sci Signal Date: 2020-08-04 Impact factor: 8.192