Literature DB >> 1846153

Transposon Tn5supF-based reverse genetic method for mutational analysis of Escherichia coli with DNAs cloned in lambda phage.

S H Phadnis1, S Kulakauskas, B R Krishnan, J Hiemstra, D E Berg.   

Abstract

An efficient method for systematic mutational analysis of the Escherichia coli genome was developed. It entails Tn5supF transposition to lambda-E. coli hybrid phage clones (Kohara library) and then transduction of recipient cells to Sup+. Essential and nonessential genes are distinguished by the ability of insertion mutant phage to form haploid versus only heterozygous partial diploid bacterial recombinants.

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Year:  1991        PMID： 1846153      PMCID： PMC207086          DOI： 10.1128/jb.173.2.896-899.1991

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  27 in total

1.  The genetic basis of hyper-synthesis of beta-galactosidase.

Authors:  T HORIUCHI; S HORIUCHI; A NOVICK
Journal:  Genetics       Date:  1963-02       Impact factor: 4.562

2.  [Duplication in the region of the phoA and lacZ genes on the Escherichia coli K-12 chromosome].

Authors:  S T Kulakauskas; G V Mitkaĭte; V G Khmeliauskaĭte
Journal:  Genetika       Date:  1990-11

3.  New method for generating deletions and gene replacements in Escherichia coli.

Authors:  C M Hamilton; M Aldea; B K Washburn; P Babitzke; S R Kushner
Journal:  J Bacteriol       Date:  1989-09       Impact factor: 3.490

4.  The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.

Authors:  Y Kohara; K Akiyama; K Isono
Journal:  Cell       Date:  1987-07-31       Impact factor: 41.582

5.  Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli.

Authors:  S C Winans; S J Elledge; J H Krueger; G C Walker
Journal:  J Bacteriol       Date:  1985-03       Impact factor: 3.490

6.  Homologous recombination in Escherichia coli: dependence on substrate length and homology.

Authors:  P Shen; H V Huang
Journal:  Genetics       Date:  1986-03       Impact factor: 4.562

7.  Mutations that affect Tn5 insertion into pBR322: importance of local DNA supercoiling.

Authors:  J K Lodge; D E Berg
Journal:  J Bacteriol       Date:  1990-10       Impact factor: 3.490

8.  Tn5supF, a 264-base-pair transposon derived from Tn5 for insertion mutagenesis and sequencing DNAs cloned in phage lambda.

Authors:  S H Phadnis; H V Huang; D E Berg
Journal:  Proc Natl Acad Sci U S A       Date:  1989-08       Impact factor: 11.205

9.  Construction of an Escherichia coli K-12 ada deletion by gene replacement in a recD strain reveals a second methyltransferase that repairs alkylated DNA.

Authors:  D E Shevell; A M Abou-Zamzam; B Demple; G C Walker
Journal:  J Bacteriol       Date:  1988-07       Impact factor: 3.490

10.  A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation.

Authors:  B Parker; M G Marinus
Journal:  Gene       Date:  1988-12-20       Impact factor: 3.688
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  5 in total

1.  cysQ, a gene needed for cysteine synthesis in Escherichia coli K-12 only during aerobic growth.

Authors:  A F Neuwald; B R Krishnan; I Brikun; S Kulakauskas; K Suziedelis; T Tomcsanyi; T S Leyh; D E Berg
Journal:  J Bacteriol       Date:  1992-01       Impact factor: 3.490

2.  Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region.

Authors:  T Yura; H Mori; H Nagai; T Nagata; A Ishihama; N Fujita; K Isono; K Mizobuchi; A Nakata
Journal:  Nucleic Acids Res       Date:  1992-07-11       Impact factor: 16.971

3.  Efficient large-scale sequencing of the Escherichia coli genome: implementation of a transposon- and PCR-based strategy for the analysis of ordered lambda phage clones.

Authors:  H Kasai; S Isono; M Kitakawa; J Mineno; H Akiyama; D M Kurnit; D E Berg; K Isono
Journal:  Nucleic Acids Res       Date:  1992-12-25       Impact factor: 16.971

4.  Direct and crossover PCR amplification to facilitate Tn5supF-based sequencing of lambda phage clones.

Authors:  B R Krishnan; D Kersulyte; I Brikun; C M Berg; D E Berg
Journal:  Nucleic Acids Res       Date:  1991-11-25       Impact factor: 16.971

5.  Efficient introduction of cloned mutant alleles into the Escherichia coli chromosome.

Authors:  S Kulakauskas; P M Wikström; D E Berg
Journal:  J Bacteriol       Date:  1991-04       Impact factor: 3.490
  5 in total

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