Natalia Cirilli1, Rita Padoan, Valeria Raia. 1. Regional Cystic Fibrosis Center, Department of Pediatrics, Ospedali Riuniti Ancona, Presidio Ospedaliero G. Salesi, Via Corridoni 11, 60123 Ancona, Italy.
Abstract
BACKGROUND: Cystic Fibrosis diagnosis is confirmed using sweat test. The aim of our study was to evaluate current techniques and methodologies in use at Italian CF Care Centres. METHODS: A series of questions related to the performance of the sweat test was collected by all CF Care Centres in Italy. Answers were compared with UK and NCCLS guidelines. RESULTS: 39/41 Centres replied to the questionnaire. A good adherence to guidelines was registered for storing samples before analysis in 90.9%, while performing CF diagnosis by at least two sweat tests, and chloride analysis were reported respectively in 100% and 75.7% of Centres. Some inconsistencies were registered for minimum acceptable sweat quantity and time to collect sweat inadequate in respectively 42.5% and 24.2% of Centres, while performing quality control procedures and referring to an external quality assessment scheme were found inadequate in respectively 54.6% and 100%. 57.6% didn't provide any appropriate analytical ranges and only 15.1% of Centres offered proper information to patients/parents. A report form, including sweat quantity, reference ranges and interpretation, was adequate only for 9.4 up to 41.4% of CF Centres. CONCLUSIONS: Our study showed areas of inconsistencies in sweat testing current practices in Italy and highlights the need for evidence based national guidelines to improve practice and management strategies.
BACKGROUND: Cystic Fibrosis diagnosis is confirmed using sweat test. The aim of our study was to evaluate current techniques and methodologies in use at Italian CF Care Centres. METHODS: A series of questions related to the performance of the sweat test was collected by all CF Care Centres in Italy. Answers were compared with UK and NCCLS guidelines. RESULTS: 39/41 Centres replied to the questionnaire. A good adherence to guidelines was registered for storing samples before analysis in 90.9%, while performing CF diagnosis by at least two sweat tests, and chloride analysis were reported respectively in 100% and 75.7% of Centres. Some inconsistencies were registered for minimum acceptable sweat quantity and time to collect sweat inadequate in respectively 42.5% and 24.2% of Centres, while performing quality control procedures and referring to an external quality assessment scheme were found inadequate in respectively 54.6% and 100%. 57.6% didn't provide any appropriate analytical ranges and only 15.1% of Centres offered proper information to patients/parents. A report form, including sweat quantity, reference ranges and interpretation, was adequate only for 9.4 up to 41.4% of CF Centres. CONCLUSIONS: Our study showed areas of inconsistencies in sweat testing current practices in Italy and highlights the need for evidence based national guidelines to improve practice and management strategies.
Authors: Maria Fátima Servidoni; Carla Cristina Souza Gomez; Fernando Augusto Lima Marson; Adyléia Aparecida Dalbo Contrera Toro; Maria Ângela Gonçalves de Oliveira Ribeiro; José Dirceu Ribeiro; Antônio Fernando Ribeiro Journal: J Bras Pneumol Date: 2017 Mar-Apr Impact factor: 2.624
Authors: Carla Cristina Souza Gomez; Fernando Augusto Lima Marson; Maria Fátima Servidoni; Antônio Fernando Ribeiro; Maria Ângela Gonçalves Oliveira Ribeiro; Veruska Acioli Lopes Gama; Eduardo Tavares Costa; José Dirceu Ribeiro; Francisco Ubaldo Vieira Junior Journal: BMC Pulm Med Date: 2018-09-14 Impact factor: 3.317
Authors: Carla Cristina Souza Gomez; Maria de Fatima Servidoni; Fernando Augusto de Lima Marson; Paulo Jose Coelho Canavezi; Adriana Mendes Vinagre; Eduardo Tavares Costa; Antonio Fernando Ribeiro; Maria Angela Gonçalves de Oliveira Ribeiro; Adyleia Aparecida Dalbo Contrera Toro; Celia Regina Pavan; Michelle Vivine Sá Dos Santos Rondon; Sonia Leticia Silva Lorena; Francisco Ubaldi Vieria; Jose Dirceu Ribeiro Journal: BMC Pulm Med Date: 2014-12-13 Impact factor: 3.317