Literature DB >> 18448688

Optimization of a laboratory-developed test utilizing roche analyte-specific reagents for detection of Staphylococcus aureus, methicillin-resistant S. aureus, and vancomycin-resistant Enterococcus species.

Maitry S Mehta1, Suzanne M Paule, Donna M Hacek, Richard B Thomson, Karen L Kaul, Lance R Peterson.   

Abstract

Nasal and perianal swab specimens were tested for detection of Staphylococcus aureus and vancomycin-resistant Enterococcus species (VRE) using a laboratory-developed real-time PCR test and microbiological cultures. The real-time PCR and culture results for S. aureus were similar. PCR had adequate sensitivity, but culture was more specific for the detection of VRE.

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Year:  2008        PMID: 18448688      PMCID: PMC2446926          DOI: 10.1128/JCM.00230-08

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  20 in total

1.  Real-time PCR can rapidly detect methicillin-susceptible and methicillin-resistant Staphylococcus aureus directly from positive blood culture bottles.

Authors:  Suzanne M Paule; Anna C Pasquariello; Richard B Thomson; Karen L Kaul; Lance R Peterson
Journal:  Am J Clin Pathol       Date:  2005-09       Impact factor: 2.493

2.  Emergence of epidemic clones of vancomycin-resistant Enterococcus faecium in Singapore.

Authors:  T H Koh; L-Y Hsu; L-L Chiu; R V T P Lin
Journal:  J Hosp Infect       Date:  2006-04-18       Impact factor: 3.926

3.  Hospital dissemination of a clonal complex 17 vanB2-containing Enterococcus faecium.

Authors:  Teresa Nebreda; Jesús Oteo; Carmen Aldea; Carmen García-Estébanez; Juan Gastelu-Iturri; Verónica Bautista; Silvia García-Cobos; José Campos
Journal:  J Antimicrob Chemother       Date:  2007-02-28       Impact factor: 5.790

4.  Performance of the BD GeneOhm methicillin-resistant Staphylococcus aureus test before and during high-volume clinical use.

Authors:  Suzanne M Paule; Donna M Hacek; Bridget Kufner; Karine Truchon; Richard B Thomson; Karen L Kaul; Ari Robicsek; Lance R Peterson
Journal:  J Clin Microbiol       Date:  2007-07-11       Impact factor: 5.948

5.  Experience with the Roche LightCycler VRE detection kit during a large outbreak of vanB2/B3 vancomycin-resistant Enterococcus faecium.

Authors:  T H Koh; R N Deepak; S Y Se-Thoe; R V T P Lin; E S C Koay
Journal:  J Antimicrob Chemother       Date:  2007-05-04       Impact factor: 5.790

6.  Modeling transmission of methicillin-resistant Staphylococcus aureus among patients admitted to a hospital.

Authors:  Janet Raboud; Refik Saskin; Andrew Simor; Mark Loeb; Karen Green; Don E Low; Allison McGeer
Journal:  Infect Control Hosp Epidemiol       Date:  2005-07       Impact factor: 3.254

7.  Impact of routine intensive care unit surveillance cultures and resultant barrier precautions on hospital-wide methicillin-resistant Staphylococcus aureus bacteremia.

Authors:  Susan S Huang; Deborah S Yokoe; Virginia L Hinrichsen; Laura S Spurchise; Rupak Datta; Irina Miroshnik; Richard Platt
Journal:  Clin Infect Dis       Date:  2006-09-14       Impact factor: 9.079

8.  Direct detection of Staphylococcus aureus from adult and neonate nasal swab specimens using real-time polymerase chain reaction.

Authors:  Suzanne M Paule; Anna C Pasquariello; Donna M Hacek; Adrienne G Fisher; Richard B Thomson; Karen L Kaul; Lance R Peterson
Journal:  J Mol Diagn       Date:  2004-08       Impact factor: 5.568

9.  Severe surgical site infection in community hospitals: epidemiology, key procedures, and the changing prevalence of methicillin-resistant Staphylococcus aureus.

Authors:  Deverick J Anderson; Daniel J Sexton; Zeina A Kanafani; Grace Auten; Keith S Kaye
Journal:  Infect Control Hosp Epidemiol       Date:  2007-07-12       Impact factor: 3.254

10.  Global spread of vancomycin-resistant Enterococcus faecium from distinct nosocomial genetic complex.

Authors:  Rob J L Willems; Janetta Top; Marga van Santen; D Ashley Robinson; Teresa M Coque; Fernando Baquero; Hajo Grundmann; Marc J M Bonten
Journal:  Emerg Infect Dis       Date:  2005-06       Impact factor: 6.883

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  3 in total

1.  Identification of Staphylococcus species directly from positive blood culture broth by use of molecular and conventional methods.

Authors:  Maitry S Mehta; Suzanne M Paule; Richard B Thomson; Karen L Kaul; Lance R Peterson
Journal:  J Clin Microbiol       Date:  2009-02-11       Impact factor: 5.948

2.  Performance characteristics of the Cepheid Xpert vanA assay for rapid identification of patients at high risk for carriage of vancomycin-resistant Enterococci.

Authors:  N Esther Babady; Kathleen Gilhuley; Diane Cianciminio-Bordelon; Yi-Wei Tang
Journal:  J Clin Microbiol       Date:  2012-09-12       Impact factor: 5.948

3.  PCR-based Approaches for the Detection of Clinical Methicillin-resistant Staphylococcus aureus.

Authors:  Ying Liu; Jiang Zhang; Yinduo Ji
Journal:  Open Microbiol J       Date:  2016-04-14
  3 in total

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