Literature DB >> 18428824

Analysis and purification of synthetic nucleic acids using HPLC.

A Andrus1, R G Kuimelis.   

Abstract

HPLC is a powerful and popular method for analyzing and purifying biomolecules. Reversed-phase HPLC allows a high-capacity method for purification, and uses volatile buffer systems that simplify product recovery. Anion-exchange HPLC provides better resolution and a more predictable elution pattern. This unit presents protocols that are optimized for HPLC of oligonucleotides. Because of the resolution limits of both reversed-phase and anion-exchange HPLC, it can be used for oligonucleotides of up to approximately 50 nt in length.

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Year:  2001        PMID： 18428824     DOI： 10.1002/0471142700.nc1005s01

Source DB:  PubMed          Journal:  Curr Protoc Nucleic Acid Chem        ISSN： 1934-9270


  9 in total

1.  Preparation and optimization of protein-DNA complexes suitable for detailed NMR studies.

Authors:  My D Sam; Robert T Clubb
Journal:  Methods Mol Biol       Date:  2012

Review 2.  Error correction in gene synthesis technology.

Authors:  Siying Ma; Ishtiaq Saaem; Jingdong Tian
Journal:  Trends Biotechnol       Date:  2011-12-28       Impact factor: 19.536

3.  Quantification bias caused by plasmid DNA conformation in quantitative real-time PCR assay.

Authors:  Chih-Hui Lin; Yu-Chieh Chen; Tzu-Ming Pan
Journal:  PLoS One       Date:  2011-12-14       Impact factor: 3.240

4.  Error correction of microchip synthesized genes using Surveyor nuclease.

Authors:  Ishtiaq Saaem; Siying Ma; Jiayuan Quan; Jingdong Tian
Journal:  Nucleic Acids Res       Date:  2011-11-29       Impact factor: 16.971

5.  Reversed-phase ion-pair liquid chromatography method for purification of duplex DNA with single base pair resolution.

Authors:  Christina L Wysoczynski; Sarah C Roemer; Vishantie Dostal; Robert M Barkley; Mair E A Churchill; Christopher S Malarkey
Journal:  Nucleic Acids Res       Date:  2013-09-05       Impact factor: 16.971

6.  Analyzing siRNA Concentration, Complexation and Stability in Cationic Dendriplexes by Stem-Loop Reverse Transcription-qPCR.

Authors:  Maximilian Neugebauer; Clara E Grundmann; Michael Lehnert; Felix von Stetten; Susanna M Früh; Regine Süss
Journal:  Pharmaceutics       Date:  2022-06-25       Impact factor: 6.525

7.  Time-Resolved Small RNA Sequencing Unravels the Molecular Principles of MicroRNA Homeostasis.

Authors:  Brian Reichholf; Veronika A Herzog; Nina Fasching; Raphael A Manzenreither; Ivica Sowemimo; Stefan L Ameres
Journal:  Mol Cell       Date:  2019-07-23       Impact factor: 17.970

8.  Error removal in microchip-synthesized DNA using immobilized MutS.

Authors:  Wen Wan; Lulu Li; Qianqian Xu; Zhefan Wang; Yuan Yao; Rongliang Wang; Jia Zhang; Haiyan Liu; Xiaolian Gao; Jiong Hong
Journal:  Nucleic Acids Res       Date:  2014-05-14       Impact factor: 16.971

9.  HPLC methods for purity evaluation of man-made single-stranded RNAs.

Authors:  Anastassia Kanavarioti
Journal:  Sci Rep       Date:  2019-01-31       Impact factor: 4.379
  9 in total

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