OBJECTIVE: The objective of the study was to determine the impact of hormones on the biomechanical properties of the vagina and its supportive tissues following surgical menopause in young vs middle-aged rats. STUDY DESIGN: Long-Evans rats (4-month virgin [n = 34], 4-month parous [n = 36], and 9-month parous [n = 34]), underwent ovariectomy (OVX) or sham surgery. OVX animals received hormones (estrogen [E2] or estrogen plus progesterone [E2 plus P4]), placebo, or a matrix metalloproteinase inhibitor (chemically modified tetracycline-8 [CMT-8]). Animals were euthanized after 8 weeks and the biomechanical properties of the vagina and supportive tissues determined. Data were analyzed using a 1-way analysis of variance and posthoc tests. RESULTS: OVX induced a rapid decline in the biomechanical properties of pelvic tissues in young but not middle-aged rats. Supplementation with E2, E2 plus P4, or CMT-8 restored tissues of young rats to control levels with no effect on middle-aged tissues. Parity did not have an impact on tissue behavior. CONCLUSION: OVX has a differential effect on the tissues of young vs middle-aged rats.
OBJECTIVE: The objective of the study was to determine the impact of hormones on the biomechanical properties of the vagina and its supportive tissues following surgical menopause in young vs middle-aged rats. STUDY DESIGN: Long-Evans rats (4-month virgin [n = 34], 4-month parous [n = 36], and 9-month parous [n = 34]), underwent ovariectomy (OVX) or sham surgery. OVX animals received hormones (estrogen [E2] or estrogen plus progesterone [E2 plus P4]), placebo, or a matrix metalloproteinase inhibitor (chemically modified tetracycline-8 [CMT-8]). Animals were euthanized after 8 weeks and the biomechanical properties of the vagina and supportive tissues determined. Data were analyzed using a 1-way analysis of variance and posthoc tests. RESULTS:OVX induced a rapid decline in the biomechanical properties of pelvic tissues in young but not middle-aged rats. Supplementation with E2, E2 plus P4, or CMT-8 restored tissues of young rats to control levels with no effect on middle-aged tissues. Parity did not have an impact on tissue behavior. CONCLUSION:OVX has a differential effect on the tissues of young vs middle-aged rats.
Authors: J Novak; L A Danielson; L J Kerchner; O D Sherwood; R J Ramirez; P A Moalli; K P Conrad Journal: J Clin Invest Date: 2001-06 Impact factor: 14.808
Authors: Pamela A Moalli; Nancy S Howden; Jerry L Lowder; Jennifer Navarro; Kristen M Debes; Steven D Abramowitch; Savio L-Y Woo Journal: Am J Obstet Gynecol Date: 2005-01 Impact factor: 8.661
Authors: Wenjun Zong; Halina M Zyczynski; Leslie A Meyn; Susan C Gordy; Pamela A Moalli Journal: Am J Obstet Gynecol Date: 2007-04 Impact factor: 8.661
Authors: Guri Rortveit; Jeanette S Brown; David H Thom; Stephen K Van Den Eeden; Jennifer M Creasman; Leslee L Subak Journal: Obstet Gynecol Date: 2007-06 Impact factor: 7.661
Authors: Andrew J Feola; Jieming Fu; Rachael Allen; Victoria Yang; Ian C Campbell; Amy Ottensmeyer; C Ross Ethier; Machelle Pardue Journal: Exp Eye Res Date: 2019-06-18 Impact factor: 3.467
Authors: Maureen E Basha; Shaohua Chang; Lara J Burrows; Jenny Lassmann; Alan J Wein; Robert S Moreland; Samuel Chacko Journal: J Sex Med Date: 2013-02-25 Impact factor: 3.802
Authors: Marianna Alperin; Andrew Feola; Robert Duerr; Pamela Moalli; Steven Abramowitch Journal: Int Urogynecol J Date: 2010-04-28 Impact factor: 2.894
Authors: Rebecca E Nakles; Maddalena Tilli Shiffert; Edgar S Díaz-Cruz; M Carla Cabrera; Maram Alotaiby; Anne M Miermont; Anna T Riegel; Priscilla A Furth Journal: Mol Endocrinol Date: 2011-02-03
Authors: Ki Hoon Ahn; Tak Kim; Jun Young Hur; Sun Haeng Kim; Kyu Wan Lee; Young Tae Kim Journal: Int Urogynecol J Date: 2010-03-23 Impact factor: 2.894
Authors: Kathleen A Connell; Marsha K Guess; Alison Tate; Vaagn Andikyan; Richard Bercik; Hugh S Taylor Journal: Menopause Date: 2009 May-Jun Impact factor: 2.953