Literature DB >> 18390669

MicroRNA-repressed mRNAs contain 40S but not 60S components.

Bingbing Wang1, Adrienne Yanez, Carl D Novina.   

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that may target more than one-third of human genes, yet the mechanisms used by miRNAs to repress translation of target mRNAs are obscure. Using a recently described cell-free assay of miRNA function, we observe that miRNA-targeted mRNAs are enriched for 40S but not 60S ribosome components. Additionally, toeprinting analysis of miRNA-targeted mRNAs demonstrates that approximately 18 nt 3' relative to the initiating AUG are protected, consistent with 40S ribosome subunits positioned at the AUG codon. Our results suggest that miRNAs repress translation initiation by preventing 60S subunit joining to miRNA-targeted mRNAs.

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Year:  2008        PMID： 18390669      PMCID： PMC2291078          DOI： 10.1073/pnas.0801102105

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  27 in total

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Review 4.  Analysis of microRNA effector functions in vitro.

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5.  MicroRNA inhibition of translation initiation in vitro by targeting the cap-binding complex eIF4F.

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Journal:  Science       Date:  2007-07-26       Impact factor: 47.728

6.  The lin-4 regulatory RNA controls developmental timing in Caenorhabditis elegans by blocking LIN-14 protein synthesis after the initiation of translation.

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7.  Lipoxygenase mRNA silencing in erythroid differentiation: The 3'UTR regulatory complex controls 60S ribosomal subunit joining.

Authors:  D H Ostareck; A Ostareck-Lederer; I N Shatsky; M W Hentze
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8.  An mRNA m7G cap binding-like motif within human Ago2 represses translation.

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9.  Drosophila miR2 induces pseudo-polysomes and inhibits translation initiation.

Authors:  Rolf Thermann; Matthias W Hentze
Journal:  Nature       Date:  2007-05-16       Impact factor: 49.962

10.  MicroRNA silencing through RISC recruitment of eIF6.

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Journal:  Nature       Date:  2007-05-16       Impact factor: 49.962
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  45 in total

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Review 2.  The mechanics of miRNA-mediated gene silencing: a look under the hood of miRISC.

Authors:  Marc R Fabian; Nahum Sonenberg
Journal:  Nat Struct Mol Biol       Date:  2012-06-05       Impact factor: 15.369

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4.  New class of microRNA targets containing simultaneous 5'-UTR and 3'-UTR interaction sites.

Authors:  Inhan Lee; Subramanian S Ajay; Jong In Yook; Hyun Sil Kim; Su Hyung Hong; Nam Hee Kim; Saravana M Dhanasekaran; Arul M Chinnaiyan; Brian D Athey
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6.  CCR4-NOT deadenylates mRNA associated with RNA-induced silencing complexes in human cells.

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Journal:  Mol Cell Biol       Date:  2010-01-11       Impact factor: 4.272

7.  MicroRNAs with a nucleolar location.

Authors:  Joan C Ritland Politz; Eric M Hogan; Thoru Pederson
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8.  Computational analysis of miRNA-mediated repression of translation: implications for models of translation initiation inhibition.

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Review 9.  Origins and Mechanisms of miRNAs and siRNAs.

Authors:  Richard W Carthew; Erik J Sontheimer
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10.  The VEGF IRESes are differentially susceptible to translation inhibition by miR-16.

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