| Literature DB >> 18379050 |
Won-Serk Kim1, So-Hyun Park, Se-Jin Ahn, Hyung-Ki Kim, Jeong-Soo Park, Ga-Young Lee, Kea-Jeung Kim, Kyu-Kwang Whang, Seung-Hee Kang, Byung-Soon Park, Jong-Hyuk Sung.
Abstract
It has been demonstrated that adipose-derived stem cells (ADSCs) secrete cytokines and exhibit diverse pharmacological actions. The present study examined the unknown pharmacological action of ADSCs regarding whitening effects. A conditioned medium of ADSCs (ADSC-CM) was harvested and the whitening effect of ADSC-CM was studied in melanoma B16 cells. ADSC-CM treatment inhibited the synthesis of melanin and the activity of tyrosinase in a dose dependent manner. To clarify the underlying mechanisms of the whitening action of ADSCs, protein levels of melanogenic proteins were measured by Western blot. Although expressions of microphthalmia-associated transcription factor and tyrosinase-related protein 2 (TRP2) remained unchanged, those of tyrosinase and TRP1 were down-regulated. Transforming growth factor-beta1 (TGF-beta 1), a potent regulator of melanogenic proteins, was neutralized by the addition of a blocking antibody to ADSC-CM, and down-regulated expression of tyrosinase and TRP1 was almost reversed. Collectively, these results indicate that secretary factors of ADSC inhibit melanin synthesis by down-regulating the expression of tyrosinase and TRP1, which are mainly mediated by TGF-beta1.Entities:
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Year: 2008 PMID： 18379050 DOI： 10.1248/bpb.31.606
Source DB: PubMed Journal: Biol Pharm Bull ISSN： 0918-6158 Impact factor: 2.233