Won-Serk Kim1, Jong-Hyuk Sung2. 1. Department of Dermatology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine , Seoul, Korea. 2. Laboratory of Skin Regeneration, Department of Applied Bioscience, CHA University , Seoul, Korea. ; Stem Cell Research Laboratory, CHA Stem Cell Institute , Seoul, Korea.
Abstract
BACKGROUND: Adipose tissue is one of the richest sources of mesenchymal stem cells that exhibit an outstanding ability to regenerate skin. THE PROBLEM: Although the anatomical sites of adipose-derived stem cells (ASCs) in the body are relatively oxygen-deficient (i.e., 1%-5% oxygen content), ASCs are usually cultured under normoxic conditions, and long-term culturing of ASCs in normoxia may induce their senescence. BASIC/CLINICAL SCIENCE ADVANCES: The review is an overview of the cellular responses of ASCs during hypoxia, which collectively increase the wound-healing potential of ASCs. Furthermore, the mechanism of action for stimulation by hypoxia (i.e., a pivotal role of reactive oxygen species and related signal pathways) will be discussed. CLINICAL CARE RELEVANCE: Hypoxia is a critical stimulatory factor for ASCs. Therefore, understanding the response and adaptation of ASCs to hypoxia may be invaluable for developing novel cell therapeutic strategies. CONCLUSION: Culturing ASCs under hypoxia may uniquely benefit proliferation, stemness, migration, and growth factor secretion. Therefore, the preconditioning of ASCs by hypoxia shows a prominent wound-healing effect in clinical use.
BACKGROUND: Adipose tissue is one of the richest sources of mesenchymal stem cells that exhibit an outstanding ability to regenerate skin. THE PROBLEM: Although the anatomical sites of adipose-derived stem cells (ASCs) in the body are relatively oxygen-deficient (i.e., 1%-5% oxygen content), ASCs are usually cultured under normoxic conditions, and long-term culturing of ASCs in normoxia may induce their senescence. BASIC/CLINICAL SCIENCE ADVANCES: The review is an overview of the cellular responses of ASCs during hypoxia, which collectively increase the wound-healing potential of ASCs. Furthermore, the mechanism of action for stimulation by hypoxia (i.e., a pivotal role of reactive oxygen species and related signal pathways) will be discussed. CLINICAL CARE RELEVANCE: Hypoxia is a critical stimulatory factor for ASCs. Therefore, understanding the response and adaptation of ASCs to hypoxia may be invaluable for developing novel cell therapeutic strategies. CONCLUSION: Culturing ASCs under hypoxia may uniquely benefit proliferation, stemness, migration, and growth factor secretion. Therefore, the preconditioning of ASCs by hypoxia shows a prominent wound-healing effect in clinical use.
Authors: Jalees Rehman; Dmitry Traktuev; Jingling Li; Stephanie Merfeld-Clauss; Constance J Temm-Grove; Jason E Bovenkerk; Carrie L Pell; Brian H Johnstone; Robert V Considine; Keith L March Journal: Circulation Date: 2004-03-01 Impact factor: 29.690
Authors: Jamie A Textor; Kaitlin C Clark; Naomi J Walker; Fabio A Aristizobal; Amir Kol; Sarah S LeJeune; Andrea Bledsoe; Arik Davidyan; Sarah N Gray; Laurie K Bohannon-Worsley; Kevin D Woolard; Dori L Borjesson Journal: Stem Cells Transl Med Date: 2017-10-24 Impact factor: 6.940
Authors: Ji Hye Kim; Sei Mee Yoon; Sun U Song; Sang Gyu Park; Won-Serk Kim; In Guk Park; Jinu Lee; Jong-Hyuk Sung Journal: Int J Mol Sci Date: 2016-08-24 Impact factor: 5.923