Literature DB >> 18369775

Expression, purification, and analysis of recombinant Drosophila Dicer-1 and Dicer-2 enzymes.

Xuecheng Ye1, Qinghua Liu.   

Abstract

RNA interference (RNAi) is a form of posttranscriptional gene silencing mediated by microRNA (miRNA) and small interfering RNA (siRNA). In Drosophila melanogaster, the RNase III enzymes Dicer-1 and Dicer-2 generate miRNA and siRNA, respectively. We describe the methods for the expression, purification, and analysis of recombinant Dicer-1 and Dicer-2 enzymes. Our studies demonstrate that Dicer-1 and Dicer-2 display different substrate specificities and ATP requirements.

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Year:  2008        PMID： 18369775      PMCID： PMC2855880          DOI： 10.1007/978-1-59745-191-8_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  19 in total

Review 1.  RNA interference.

Authors:  Gregory J Hannon
Journal:  Nature       Date:  2002-07-11       Impact factor: 49.962

2.  Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans.

Authors:  R F Ketting; S E Fischer; E Bernstein; T Sijen; G J Hannon; R H Plasterk
Journal:  Genes Dev       Date:  2001-10-15       Impact factor: 11.361

Review 3.  Post-transcriptional gene silencing by siRNAs and miRNAs.

Authors:  Witold Filipowicz; Lukasz Jaskiewicz; Fabrice A Kolb; Ramesh S Pillai
Journal:  Curr Opin Struct Biol       Date:  2005-06       Impact factor: 6.809

Review 4.  Silence from within: endogenous siRNAs and miRNAs.

Authors:  Erik J Sontheimer; Richard W Carthew
Journal:  Cell       Date:  2005-07-15       Impact factor: 41.582

5.  Micro RNAs in animal development.

Authors:  Ronald H A Plasterk
Journal:  Cell       Date:  2006-03-10       Impact factor: 41.582

6.  Structural basis for double-stranded RNA processing by Dicer.

Authors:  Ian J Macrae; Kaihong Zhou; Fei Li; Adrian Repic; Angela N Brooks; W Zacheus Cande; Paul D Adams; Jennifer A Doudna
Journal:  Science       Date:  2006-01-13       Impact factor: 47.728

7.  Role for a bidentate ribonuclease in the initiation step of RNA interference.

Authors:  E Bernstein; A A Caudy; S M Hammond; G J Hannon
Journal:  Nature       Date:  2001-01-18       Impact factor: 49.962

8.  Dicer-1 and R3D1-L catalyze microRNA maturation in Drosophila.

Authors:  Feng Jiang; Xuecheng Ye; Xiang Liu; Lauren Fincher; Dennis McKearin; Qinghua Liu
Journal:  Genes Dev       Date:  2005-06-28       Impact factor: 11.361

Review 9.  Oncomirs - microRNAs with a role in cancer.

Authors:  Aurora Esquela-Kerscher; Frank J Slack
Journal:  Nat Rev Cancer       Date:  2006-04       Impact factor: 60.716

10.  Processing of pre-microRNAs by the Dicer-1-Loquacious complex in Drosophila cells.

Authors:  Kuniaki Saito; Akira Ishizuka; Haruhiko Siomi; Mikiko C Siomi
Journal:  PLoS Biol       Date:  2005-05-24       Impact factor: 8.029
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  8 in total

1.  Hairpin dsRNA does not trigger RNA interference in Candida albicans cells.

Authors:  Janet F Staab; Theodore C White; Kieren A Marr
Journal:  Yeast       Date:  2010-08-24       Impact factor: 3.239

2.  Dicer's helicase domain discriminates dsRNA termini to promote an altered reaction mode.

Authors:  Noah C Welker; Tuhin S Maity; Xuecheng Ye; P Joseph Aruscavage; Ammie A Krauchuk; Qinghua Liu; Brenda L Bass
Journal:  Mol Cell       Date:  2011-03-04       Impact factor: 17.970

3.  MicroRNA-like viral small RNA from Dengue virus 2 autoregulates its replication in mosquito cells.

Authors:  Mazhar Hussain; Sassan Asgari
Journal:  Proc Natl Acad Sci U S A       Date:  2014-02-03       Impact factor: 11.205

4.  Viral suppressors of RNAi employ a rapid screening mode to discriminate viral RNA from cellular small RNA.

Authors:  Mohamed Fareh; Jasper van Lopik; Iason Katechis; Alfred W Bronkhorst; Anna C Haagsma; Ronald P van Rij; Chirlmin Joo
Journal:  Nucleic Acids Res       Date:  2018-04-06       Impact factor: 16.971

5.  Derivation and characterization of Dicer- and microRNA-deficient human cells.

Authors:  Hal P Bogerd; Adam W Whisnant; Edward M Kennedy; Omar Flores; Bryan R Cullen
Journal:  RNA       Date:  2014-04-22       Impact factor: 4.942

6.  Structural and functional insights into the fly microRNA biogenesis factor Loquacious.

Authors:  Leonhard Jakob; Thomas Treiber; Nora Treiber; Alexander Gust; Kevin Kramm; Kerrin Hansen; Mathias Stotz; Ludwig Wankerl; Franz Herzog; Stefan Hannus; Dina Grohmann; Gunter Meister
Journal:  RNA       Date:  2016-01-14       Impact factor: 4.942

7.  Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation.

Authors:  Olga Coll; Tanit Guitart; Ana Villalba; Catherine Papin; Martine Simonelig; Fátima Gebauer
Journal:  RNA       Date:  2018-01-09       Impact factor: 4.942

8.  Drosophila melanogaster Acetyl-CoA-carboxylase sustains a fatty acid-dependent remote signal to waterproof the respiratory system.

Authors:  Jean-Philippe Parvy; Laura Napal; Thomas Rubin; Mickael Poidevin; Laurent Perrin; Claude Wicker-Thomas; Jacques Montagne
Journal:  PLoS Genet       Date:  2012-08-30       Impact factor: 5.917
  8 in total

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