| Literature DB >> 18360908 |
Yongbo Yang1, Chengxiang Wu, Jianguo Wu, Vivek R Nerurkar, Richard Yanagihara, Yuanan Lu.
Abstract
West Nile virus (WNV) has been responsible for the largest outbreaks of arboviral encephalitis in U.S. history. No specific drug is currently available for the effective treatment of WNV infection. To exploit RNA interference as a potential therapeutic approach, a Moloney murine leukemia virus-based retrovirus vector was used to effectively deliver WNV-specific small interfering RNA (siRNA) into human neuroblastoma HTB-11 cells. Viral plaque assays demonstrated that transduced cells were significantly refractory to WNV replication, as compared to untransduced control cells (P < 0.05), which correlated with the reduced expression of target viral genes and respective viral proteins. Therefore, retrovirus-mediated delivery of siRNA for gene silencing can be used to study the specific functions of viral genes associated with replication and may have potential therapeutic applications.Entities:
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Year: 2008 PMID： 18360908 PMCID： PMC2825143 DOI： 10.1002/jmv.21164
Source DB: PubMed Journal: J Med Virol ISSN： 0146-6615 Impact factor: 2.327