Literature DB >> 18318714

Quantification of mcrA by fluorescent PCR in methanogenic and methanotrophic microbial communities.

Takuro Nunoura1, Hanako Oida, Junichi Miyazaki, Ai Miyashita, Hiroyuki Imachi, Ken Takai.   

Abstract

A quantitative fluorogenic PCR method for detecting methanogenic and methanotrophic orders was established using a refined primer set for the methyl coenzyme M reductase subunit A gene (mcrA). The method developed was applied to several microbial communities in which diversity and abundance of methanogens or anaerobic methanotrophs (ANMEs) was identified by 16S rRNA gene clone analysis, and strong correlations between the copy numbers of mcrA with those of archaeal 16S rRNA genes in the communities were observed. The assay can be applied to detecting and assessing the abundance of methanogens and/or ANMEs in anoxic environments that could not be detected by 16S rRNA gene sequence analyses.

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Year:  2008        PMID： 18318714     DOI： 10.1111/j.1574-6941.2008.00451.x

Source DB:  PubMed          Journal:  FEMS Microbiol Ecol        ISSN： 0168-6496            Impact factor:   4.194


  28 in total

1.  Spatial distribution of viruses associated with planktonic and attached microbial communities in hydrothermal environments.

Authors:  Yukari Yoshida-Takashima; Takuro Nunoura; Hiromi Kazama; Takuroh Noguchi; Kazuhiro Inoue; Hironori Akashi; Toshiro Yamanaka; Tomohiro Toki; Masahiro Yamamoto; Yasuo Furushima; Yuichiro Ueno; Hiroyuki Yamamoto; Ken Takai
Journal:  Appl Environ Microbiol       Date:  2011-12-30       Impact factor: 4.792

2.  Primer evaluation and adaption for cost-efficient SYBR Green-based qPCR and its applicability for specific quantification of methanogens.

Authors:  Christoph Reitschuler; Philipp Lins; Paul Illmer
Journal:  World J Microbiol Biotechnol       Date:  2013-08-06       Impact factor: 3.312

3.  Molecular characterization of potential nitrogen fixation by anaerobic methane-oxidizing archaea in the methane seep sediments at the number 8 Kumano Knoll in the Kumano Basin, offshore of Japan.

Authors:  Junichi Miyazaki; Ryosaku Higa; Tomohiro Toki; Juichiro Ashi; Urumu Tsunogai; Takuro Nunoura; Hiroyuki Imachi; Ken Takai
Journal:  Appl Environ Microbiol       Date:  2009-09-25       Impact factor: 4.792

4.  Comparative Analyses of Methanogenic and Methanotrophic Communities Between Two Different Water Regimes in Controlled Wetlands on the Qinghai-Tibetan Plateau, China.

Authors:  Hongpeng Cui; Xin Su; Shiping Wei; Youhai Zhu; Zhenquan Lu; Yanfa Wang; Yuejiao Li; Hui Liu; Shuai Zhang; Shouji Pang
Journal:  Curr Microbiol       Date:  2017-11-29       Impact factor: 2.188

5.  Pyrosequencing of mcrA and archaeal 16S rRNA genes reveals diversity and substrate preferences of methanogen communities in anaerobic digesters.

Authors:  David Wilkins; Xiao-Ying Lu; Zhiyong Shen; Jiapeng Chen; Patrick K H Lee
Journal:  Appl Environ Microbiol       Date:  2014-11-07       Impact factor: 4.792

6.  Defining boundaries for the distribution of microbial communities beneath the sediment-buried, hydrothermally active seafloor.

Authors:  Katsunori Yanagawa; Akira Ijiri; Anja Breuker; Sanae Sakai; Youko Miyoshi; Shinsuke Kawagucci; Takuroh Noguchi; Miho Hirai; Axel Schippers; Jun-Ichiro Ishibashi; Yoshihiro Takaki; Michinari Sunamura; Tetsuro Urabe; Takuro Nunoura; Ken Takai
Journal:  ISME J       Date:  2016-10-18       Impact factor: 10.302

7.  Shifts of methanogenic communities in response to permafrost thaw results in rising methane emissions and soil property changes.

Authors:  Shiping Wei; Hongpeng Cui; Youhai Zhu; Zhenquan Lu; Shouji Pang; Shuai Zhang; Hailiang Dong; Xin Su
Journal:  Extremophiles       Date:  2018-02-10       Impact factor: 2.395

8.  The Water Hyacinth Microbiome: Link Between Carbon Turnover and Nutrient Cycling.

Authors:  Marcelo P Ávila; Ernandes S Oliveira-Junior; Mariana P Reis; Eric R Hester; Cristiane Diamantino; Annelies J Veraart; Leon P M Lamers; Sarian Kosten; Andréa M A Nascimento
Journal:  Microb Ecol       Date:  2019-02-01       Impact factor: 4.552

9.  Extracellular quinones affecting methane production and methanogenic community in paddy soil.

Authors:  Jielong Xu; Li Zhuang; Guiqin Yang; Yong Yuan; Shungui Zhou
Journal:  Microb Ecol       Date:  2013-08-04       Impact factor: 4.552

10.  Estimating Population Turnover Rates by Relative Quantification Methods Reveals Microbial Dynamics in Marine Sediment.

Authors:  Richard Kevorkian; Jordan T Bird; Alexander Shumaker; Karen G Lloyd
Journal:  Appl Environ Microbiol       Date:  2017-12-15       Impact factor: 4.792
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