Literature DB >> 643612

Oestradiol-receptor complexes in subnuclear fractions of rat uterine tissue.

W de Boer, J de Vries, E Mulder, H J van der Molen.   

Abstract

The subnuclear distribution of 3H-oestradiol-receptor complexes was studied in uterine tissue of ovariectomized adult rats. Nuclei were sonically disrupted and 8 different subnuclear fractions were isolated by discontinuous sucrose density gradient centrifugation. 3H-Oestradiol-receptor complexes, measured by hydroxylapatite column chromatography, were localized in a light chromatin fraction as well as in a heavy chromatin fraction. Using the hydroxylapatite chromatography technique it was possible to demonstrate three classes of oestradiol-receptor complexes which differ in affinity for the chromatin. Oestradiol-receptor complexes with a high affinity for the chromatin were predominantly localized in the heavy chromatin fraction, whereas complexes with a lower affinity for their acceptor sites were present in the lighter chromatin fraction.

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Year:  1978        PMID： 643612      PMCID： PMC341963          DOI： 10.1093/nar/5.1.87

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  39 in total

1.  ACTIVE AND INACTIVE REGIONS OF NUCLEAR CHROMATIN AS REVEALED BY ELECTRON MICROSCOPE AUTORADIOGRAPHY.

Authors:  V C LITTAU; V G ALLFREY; J H FRENSTER; A E MIRSKY
Journal:  Proc Natl Acad Sci U S A       Date:  1964-07       Impact factor: 11.205

2.  REPRESSED AND ACTIVE CHROMATIN ISOLATED FROM INTERPHASE LYMPHOCYTES.

Authors:  J H FRENSTER; V G ALLFREY; A E MIRSKY
Journal:  Proc Natl Acad Sci U S A       Date:  1963-12       Impact factor: 11.205

3.  POSSIBLE SYNTHESIS OF POLYRIBONUCLEOTIDES OF KNOWN BASE-TRIPLET SEQUENCES.

Authors:  R W MASTER
Journal:  Nature       Date:  1965-04-03       Impact factor: 49.962

4.  The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation.

Authors:  A FLECK; H N MUNRO
Journal:  Biochim Biophys Acta       Date:  1962-05-14

5.  Differential rate in RNA synthesis between euchromatin and heterochromatin.

Authors:  T C HSU
Journal:  Exp Cell Res       Date:  1962-08       Impact factor: 3.905

6.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

7.  Isolation of a chromatin fraction from calf endometrium highly enriched in estradiol binding sites.

Authors:  J M Sala-Trepat; U Hibner; C Vallet-Strouvé
Journal:  Nucleic Acids Res       Date:  1977-03       Impact factor: 16.971

8.  Differential distribution of oestrogen receptors in subfractions of oviduct chromatin.

Authors:  K Hemminki
Journal:  Acta Endocrinol (Copenh)       Date:  1977-01

9.  The distirubtion of active genes (globin) and inactive genes (keratin) in fractionated chicken erythroid chromatin.

Authors:  P Krieg; J R Wells
Journal:  Biochemistry       Date:  1976-10-19       Impact factor: 3.162

10.  Comparative study of nuclear binding sites for oestradiol in rat testicular and uterine tissue. Determination of low amounts of specific binding site by an [3H] oestradiol-exchange method.

Authors:  W de Boer; J de Vries; E Mulder; H J van der Molen
Journal:  Biochem J       Date:  1977-02-15       Impact factor: 3.857
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  1 in total

1.  Cortisol-induced stimulation of nuclear matrix protein phosphorylation.

Authors:  L Sevaljević; N Brajanović; D Trajković
Journal:  Mol Biol Rep       Date:  1982-11-30       Impact factor: 2.316
  1 in total

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