Literature DB >> 182529

Rapid purification of lactate dehydrogenase X from mouse testes by two steps of affinity chromatography on oxamate-sepharose.

H Spielmann, H G Eibs, C Mentzel.   

Abstract

At concentrations of 200 muM NADH and 0.5 M NaCl LDH-X is separated from the other LDH isozymes of mouse testes on oxamate-sepharose. In a second step LDH-X is bound to the same matrix at lower NADH and NaCl concentrations and the pure enzyme can subsequently be eluted.

Entities:  

Keywords:  Animals, Laboratory; Biology; Clinical Research; Examinations And Diagnoses; Genitalia; Genitalia, Male; In Vitro; Laboratory Examinations And Diagnoses; Laboratory Procedures; Physiology; Research Methodology; Testis; Urogenital System

Mesh：

Substances：

Year:  1976        PMID： 182529     DOI： 10.1007/bf01933984

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  11 in total

1.  Pregnancy suppression by an antiserum to the sperm specific lactate dehydrogenase.

Authors:  E Goldberg; J Lerum
Journal:  Science       Date:  1972-05-12       Impact factor: 47.728

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Affinity chromatographic differentiation of lactate dehydrogenase isoenzymes on the basis of differential abortive complex formation.

Authors:  P O'Carra; S Barry; E Corcoran
Journal:  FEBS Lett       Date:  1974-07-15       Impact factor: 4.124

4.  Isolation and properties of lactate dehydrogenase isozyme X from Swiss mice.

Authors:  C Wong; R Yañez; D M Brown; A Dickey; M E Parks; R W McKee
Journal:  Arch Biochem Biophys       Date:  1971-10       Impact factor: 4.013

5.  Immunological impairment of pregnancy in mice by lactate dehydrogenase-X.

Authors:  J E Lerum; E Goldberg
Journal:  Biol Reprod       Date:  1974-08       Impact factor: 4.285

6.  Infertility in female rabbits immunized with lactate dehydrogenase X.

Authors:  E Goldberg
Journal:  Science       Date:  1973-08-03       Impact factor: 47.728

7.  Binding of antibody to LDH-X by the mouse blastocyst. 1.

Authors:  M Bené; E Goldberg
Journal:  J Exp Zool       Date:  1974-08

8.  The separation of lactate dehydrogenase X from other lactate dehydrogenase isozymes of mouse testes by affinity chromatography.

Authors:  H Spielmann; R P Erickson; C J Epstein
Journal:  FEBS Lett       Date:  1973-09-01       Impact factor: 4.124

9.  Biochemical development of preimplantation mouse embryos: in vivo activities of fructose 1,6-diphosphate aldolase, glucose 6-phosphate dehydrogenase, malate dehydrogenase, and lactate dehydrogenase.

Authors:  C J Epstein; E A Wegienka; C W Smith
Journal:  Biochem Genet       Date:  1969-06       Impact factor: 1.890

10.  Amino acid composition and properties of crystalline lactate dehydrogenase X from mouse testes.

Authors:  E Goldberg
Journal:  J Biol Chem       Date:  1972-04-10       Impact factor: 5.157
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