PURPOSE: Near-infrared fluorescent probes for amyloid-beta (Abeta) are an exciting option for molecular imaging in Alzheimer's disease research and may translate to clinical diagnostics. However, Abeta-targeted optical probes often suffer from poor specificity and slow clearance from the brain. We are designing smart optical probes that emit characteristic fluorescence signal only when bound to Abeta. METHODS: We synthesized a family of dyes and tested Abeta-binding sensitivity with fluorescence spectroscopy and tissue-staining. RESULTS: Select compounds exhibited Abeta-dependent changes in fluorescence quantum yield, lifetime, and emission spectra that may be imaged microscopically or in vivo using new lifetime and spectral fluorescence imaging techniques. CONCLUSION: Smart optical probes that turn on when bound to Abeta will improve amyloid detection and may enable quantitative molecular imaging in vivo.
PURPOSE: Near-infrared fluorescent probes for amyloid-beta (Abeta) are an exciting option for molecular imaging in Alzheimer's disease research and may translate to clinical diagnostics. However, Abeta-targeted optical probes often suffer from poor specificity and slow clearance from the brain. We are designing smart optical probes that emit characteristic fluorescence signal only when bound to Abeta. METHODS: We synthesized a family of dyes and tested Abeta-binding sensitivity with fluorescence spectroscopy and tissue-staining. RESULTS: Select compounds exhibited Abeta-dependent changes in fluorescence quantum yield, lifetime, and emission spectra that may be imaged microscopically or in vivo using new lifetime and spectral fluorescence imaging techniques. CONCLUSION: Smart optical probes that turn on when bound to Abeta will improve amyloid detection and may enable quantitative molecular imaging in vivo.
Authors: Kooresh Shoghi-Jadid; Gary W Small; Eric D Agdeppa; Vladimir Kepe; Linda M Ercoli; Prabha Siddarth; Stephen Read; Nagichettiar Satyamurthy; Andrej Petric; Sung-Cheng Huang; Jorge R Barrio Journal: Am J Geriatr Psychiatry Date: 2002 Jan-Feb Impact factor: 4.105
Authors: Henry Engler; Anton Forsberg; Ove Almkvist; Gunnar Blomquist; Emma Larsson; Irina Savitcheva; Anders Wall; Anna Ringheim; Bengt Långström; Agneta Nordberg Journal: Brain Date: 2006-07-19 Impact factor: 13.501
Authors: D Games; D Adams; R Alessandrini; R Barbour; P Berthelette; C Blackwell; T Carr; J Clemens; T Donaldson; F Gillespie Journal: Nature Date: 1995-02-09 Impact factor: 49.962
Authors: Masahiro Ono; Alan Wilson; Jose Nobrega; David Westaway; Paul Verhoeff; Zhi-Ping Zhuang; Mei-Ping Kung; Hank F Kung Journal: Nucl Med Biol Date: 2003-08 Impact factor: 2.408
Authors: Victoria A Lawson; Cathryn L Haigh; Blaine Roberts; Vijaya B Kenche; Helen M J Klemm; Colin L Masters; Steven J Collins; Kevin J Barnham; Simon C Drew Journal: ACS Chem Neurosci Date: 2010-09-30 Impact factor: 4.418
Authors: Matteo Staderini; Suzana Aulić; Manuela Bartolini; Hoang Ngoc Ai Tran; Víctor González-Ruiz; Daniel I Pérez; Nieves Cabezas; Ana Martínez; M Antonia Martín; Vincenza Andrisano; Giuseppe Legname; J Carlos Menéndez; Maria Laura Bolognesi Journal: ACS Med Chem Lett Date: 2012-12-28 Impact factor: 4.345