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Literature DB >> 18234878

Identification of Staphylococcus aureus and determination of methicillin resistance directly from positive blood cultures by isothermal amplification and a disposable detection device.

James Goldmeyer¹, Haijing Li, Melinda McCormac, Steve Cook, Charles Stratton, Bertrand Lemieux, Huimin Kong, Wen Tang, Yi-Wei Tang.

Abstract

A simple, rapid, and user-friendly procedure has been developed to identify Staphylococcus aureus and determine its methicillin resistance directly from gram-positive cocci in cluster-containing blood culture medium. The specimens were diluted and heated prior to amplification of the nuc and mecA genes with isothermal helicase-dependent amplification. Amplicons were detected using a disposable detection device. The analytical sensitivity of the assays was 50 CFU per reaction, and the clinical sensitivity and specificity were both 100% for S. aureus detection and 100% and 98% for methicillin resistance determination, respectively.

Entities: Chemical Species

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Year: 2008 PMID： 18234878 PMCID： PMC2292917 DOI： 10.1128/JCM.02234-07

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

14 in total

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Review 2. Methicillin-resistant Staphylococcus aureus: an evolutionary, epidemiologic, and therapeutic odyssey.

Authors: Stan Deresinski
Journal: Clin Infect Dis Date: 2005-01-24 Impact factor: 9.079

3. Real-time PCR can rapidly detect methicillin-susceptible and methicillin-resistant Staphylococcus aureus directly from positive blood culture bottles.

Authors: Suzanne M Paule; Anna C Pasquariello; Richard B Thomson; Karen L Kaul; Lance R Peterson
Journal: Am J Clin Pathol Date: 2005-09 Impact factor: 2.493

4. Characterization of a thermostable UvrD helicase and its participation in helicase-dependent amplification.

Authors: Lixin An; Wen Tang; Tamara A Ranalli; Hyun-Jin Kim; Jamie Wytiaz; Huimin Kong
Journal: J Biol Chem Date: 2005-06-13 Impact factor: 5.157

5. Rapid identification of methicillin-resistant Staphylococcus aureus from positive blood cultures by real-time fluorescence PCR.

Authors: T Y Tan; S Corden; R Barnes; B Cookson
Journal: J Clin Microbiol Date: 2001-12 Impact factor: 5.948

6. Rapid identification of staphylococcal strains from positive-testing blood culture bottles by internal transcribed spacer PCR followed by microchip gel electrophoresis.

Authors: Shin-Ichi Fujita; Yasuko Senda; Thikako Iwagami; Takuma Hashimoto
Journal: J Clin Microbiol Date: 2005-03 Impact factor: 5.948

7. Development of a novel one-tube isothermal reverse transcription thermophilic helicase-dependent amplification platform for rapid RNA detection.

Authors: James Goldmeyer; Huimin Kong; Wen Tang
Journal: J Mol Diagn Date: 2007-11 Impact factor: 5.568

8. Rapid identification of Staphylococcus aureus and the mecA gene from BacT/ALERT blood culture bottles by using the LightCycler system.

Authors: Nabin K Shrestha; Marion J Tuohy; Gerri S Hall; Carlos M Isada; Gary W Procop
Journal: J Clin Microbiol Date: 2002-07 Impact factor: 5.948

9. Rapid detection of methicillin-resistant staphylococci by real-time PCR directly from positive blood culture bottles.

Authors: S Gröbner; V A J Kempf
Journal: Eur J Clin Microbiol Infect Dis Date: 2007-10 Impact factor: 3.267

10. StaphPlex system for rapid and simultaneous identification of antibiotic resistance determinants and Panton-Valentine leukocidin detection of staphylococci from positive blood cultures.

Authors: Yi-Wei Tang; Abdullah Kilic; Qunying Yang; Sigrid K McAllister; Haijing Li; Rebecca S Miller; Melinda McCormac; Karen D Tracy; Charles W Stratton; Jian Han; Brandi Limbago
Journal: J Clin Microbiol Date: 2007-04-19 Impact factor: 5.948

21 in total

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Authors: M R Williams; R D Stedtfeld; H Waseem; T Stedtfeld; B Upham; W Khalife; B Etchebarne; M Hughes; J M Tiedje; S A Hashsham
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3. Helicase-Dependent Isothermal Amplification of DNA and RNA by Using Self-Avoiding Molecular Recognition Systems.

Authors: Zunyi Yang; Chris McLendon; Daniel Hutter; Kevin M Bradley; Shuichi Hoshika; Carole B Frye; Steven A Benner
Journal: Chembiochem Date: 2015-05-07 Impact factor: 3.164

4. Evaluation of loop-mediated isothermal amplification for the rapid identification of bacteria and resistance determinants in positive blood cultures.

Authors: J Rödel; J A Bohnert; S Stoll; L Wassill; B Edel; M Karrasch; B Löffler; W Pfister
Journal: Eur J Clin Microbiol Infect Dis Date: 2017-01-06 Impact factor: 3.267

5. A probe-based method for confirmation of methicillin-resistant Staphylococcus aureus and detection of Panton-Valentine leukocidin and tst virulence genes.

Authors: Ashok Srinivasan; Matthew J Bankowski; Steven E Seifried; Sadao Jinno; Rosalie Perkins; Seema Singh; Claire Ying; Alan D Tice; Wesley Kim; Randall T Hayden
Journal: Diagn Microbiol Infect Dis Date: 2011-06-11 Impact factor: 2.803

6. Comparative clinical evaluation of the IsoAmp(®) HSV Assay with ELVIS(®) HSV culture/ID/typing test system for the detection of herpes simplex virus in genital and oral lesions.

Authors: Nancy S Miller; Belinda Yen-Lieberman; Melinda D Poulter; Yi-Wei Tang; Paul A Granato
Journal: J Clin Virol Date: 2012-05-20 Impact factor: 3.168

7. Detection of severe fever with thrombocytopenia syndrome virus by reverse transcription-cross-priming amplification coupled with vertical flow visualization.

Authors: Lunbiao Cui; Yiyue Ge; Xian Qi; Gaolian Xu; Haijing Li; Kangchen Zhao; Bin Wu; Zhiyang Shi; Xiling Guo; Lin Hu; Qimin You; Li-Hong Zhang; Alexander N Freiberg; Xuejie Yu; Hua Wang; Minghao Zhou; Yi-Wei Tang
Journal: J Clin Microbiol Date: 2012-09-19 Impact factor: 5.948