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Literature DB >> 18228314

Analysis of eukaryotic translation in purified and semipurified systems.

Abstract

Much of the current understanding of the sequential steps involved in translation initiation has been obtained using sucrose gradients to isolate ribosomes and ribosomal subunits, as described here. These purified components are combined with purified translation factors to analyze the formation of intermediates in translation initiation and the roles of the translation factors in vitro.

Entities: Chemical

Mesh：

Substances：
Cell Extracts

Year: 2001 PMID： 18228314 DOI： 10.1002/0471143030.cb1109s08

Source DB: PubMed Journal: Curr Protoc Cell Biol ISSN： 1934-2616

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Cited

12 in total

1. S6 phosphorylation-independent pathways regulate translation of 5'-terminal oligopyrimidine tract-containing mRNAs in differentiating hematopoietic cells.

Authors: Diane Barth-Baus; Carl A Stratton; Lou Parrott; Howard Myerson; Oded Meyuhas; Dennis J Templeton; Gary E Landreth; Jack O Hensold
Journal: Nucleic Acids Res Date: 2002-05-01 Impact factor: 16.971

2. Structure of the 40S-ABCE1 post-splitting complex in ribosome recycling and translation initiation.

Authors: André Heuer; Milan Gerovac; Christian Schmidt; Simon Trowitzsch; Anne Preis; Peter Kötter; Otto Berninghausen; Thomas Becker; Roland Beckmann; Robert Tampé
Journal: Nat Struct Mol Biol Date: 2017-04-03 Impact factor: 15.369

3. Repression of VEGFA by CA-rich element-binding microRNAs is modulated by hnRNP L.

Authors: Faegheh Jafarifar; Peng Yao; Sandeepa M Eswarappa; Paul L Fox
Journal: EMBO J Date: 2011-02-22 Impact factor: 11.598

4. Identification of an mRNP complex regulating tumorigenesis at the translational elongation step.

Authors: George S Hussey; Arindam Chaudhury; Andrea E Dawson; Daniel J Lindner; Charlotte R Knudsen; Matthew C J Wilce; William C Merrick; Philip H Howe
Journal: Mol Cell Date: 2011-02-18 Impact factor: 17.970

5. The Pim-1 protein kinase is an important regulator of MET receptor tyrosine kinase levels and signaling.

Authors: Bo Cen; Ying Xiong; Jin H Song; Sandeep Mahajan; Rachel DuPont; Kristen McEachern; Daniel J DeAngelo; Jorge E Cortes; Mark D Minden; Allen Ebens; Alice Mims; Amanda C LaRue; Andrew S Kraft
Journal: Mol Cell Biol Date: 2014-04-28 Impact factor: 4.272

Analysis of eukaryotic translation in purified and semipurified systems.

1. S6 phosphorylation-independent pathways regulate translation of 5'-terminal oligopyrimidine tract-containing mRNAs in differentiating hematopoietic cells.

2. Structure of the 40S-ABCE1 post-splitting complex in ribosome recycling and translation initiation.

3. Repression of VEGFA by CA-rich element-binding microRNAs is modulated by hnRNP L.

4. Identification of an mRNP complex regulating tumorigenesis at the translational elongation step.

5. The Pim-1 protein kinase is an important regulator of MET receptor tyrosine kinase levels and signaling.

6. Polysome Analysis.

7. Cyclin B Translation Depends on mTOR Activity after Fertilization in Sea Urchin Embryos.

8. A stress-responsive RNA switch regulates VEGFA expression.

9. Bouvardin is a Radiation Modulator with a Novel Mechanism of Action.

10. Establishment of a TGFβ-induced post-transcriptional EMT gene signature.