Literature DB >> 28603752

Polysome Analysis.

Dipak Kumar Poria1, Partho Sarothi Ray1.   

Abstract

Polysome analysis is a method to separate mRNAs from a cell into actively translating and non-translating fractions depending on their association with polysomes. By this protocol, cell lysates are fractionated by sucrose density gradient ultracentrifugation. Free mRNA fraction and various ribosomal fractions, such as 40S, 60S, monosomes and polysomes are collected by fractionation. Association of particular mRNAs with these fractions is detected by reverse transcription - PCR to investigate the translational state of the mRNA.

Entities:  

Keywords:  Polysome; Ribosome fractionation; Sucrose density gradient; Translation; Ultracentrifugation; mRNA

Year:  2017        PMID： 28603752      PMCID： PMC5466151          DOI： 10.21769/BioProtoc.2192

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  3 in total

1.  Analysis of eukaryotic translation in purified and semipurified systems.

Authors:  W C Merrick; J O Hensold
Journal:  Curr Protoc Cell Biol       Date:  2001-05

2.  RNA-binding protein HuR sequesters microRNA-21 to prevent translation repression of proinflammatory tumor suppressor gene programmed cell death 4.

Authors:  D K Poria; A Guha; I Nandi; P S Ray
Journal:  Oncogene       Date:  2015-07-20       Impact factor: 9.867

3.  A stress-responsive RNA switch regulates VEGFA expression.

Authors:  Partho Sarothi Ray; Jie Jia; Peng Yao; Mithu Majumder; Maria Hatzoglou; Paul L Fox
Journal:  Nature       Date:  2008-12-21       Impact factor: 49.962
  3 in total
  2 in total

1.  Efficient and Rapid Analysis of Polysomes and Ribosomal Subunits in Cells and Tissues Using Ribo Mega-SEC.

Authors:  Harunori Yoshikawa; Ramasubramanian Sundaramoorthy; Daniel Mariyappa; Hao Jiang; Angus I Lamond
Journal:  Bio Protoc       Date:  2021-08-05

2.  Post-Transcriptional Dynamics is Involved in Rapid Adaptation to Hypergravity in Jurkat T Cells.

Authors:  Christian Vahlensieck; Cora S Thiel; Daniel Pöschl; Timothy Bradley; Sonja Krammer; Beatrice Lauber; Jennifer Polzer; Oliver Ullrich
Journal:  Front Cell Dev Biol       Date:  2022-07-04
  2 in total

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