OBJECTIVES: An in vivo rabbit model was used to study the effect of 3 hours of experimentally induced phonation on messenger RNA expression of the normal vocal fold. STUDY DESIGN: Prospective; animal model. SUBJECTS AND METHODS: Ten rabbits received experimental phonation for 3 hours, followed by 1 hour of recovery. A separate group of 5 rabbits served as no-phonation controls. We measured messenger RNA expression of matrix metalloproteinase-1, MMP-9, and interleukin-1beta using real-time reverse-transcribed polymerase chain reaction. Gene expression ratios from phonation and control animals were assessed with the Mann-Whitney U test. RESULTS: Phonation (77 +/- 3 dB; 429 +/- 141 Hz) resulted in increased matrix metalloproteinase-1 gene expression from rabbits receiving experimental phonation compared with controls, and a nonsignificant increase in matrix metalloproteinase-9 and interleukin-1beta gene expression. CONCLUSION: Matrix metalloproteinases play a role in maintaining tissue homeostasis. Investigation of cellular responses to experimental phonation may provide insight into how matrix metalloproteinases and other extracellular matrices contribute to maintenance of the vocal fold and development of pathology.
OBJECTIVES: An in vivo rabbit model was used to study the effect of 3 hours of experimentally induced phonation on messenger RNA expression of the normal vocal fold. STUDY DESIGN: Prospective; animal model. SUBJECTS AND METHODS: Ten rabbits received experimental phonation for 3 hours, followed by 1 hour of recovery. A separate group of 5 rabbits served as no-phonation controls. We measured messenger RNA expression of matrix metalloproteinase-1, MMP-9, and interleukin-1beta using real-time reverse-transcribed polymerase chain reaction. Gene expression ratios from phonation and control animals were assessed with the Mann-Whitney U test. RESULTS: Phonation (77 +/- 3 dB; 429 +/- 141 Hz) resulted in increased matrix metalloproteinase-1 gene expression from rabbits receiving experimental phonation compared with controls, and a nonsignificant increase in matrix metalloproteinase-9 and interleukin-1beta gene expression. CONCLUSION: Matrix metalloproteinases play a role in maintaining tissue homeostasis. Investigation of cellular responses to experimental phonation may provide insight into how matrix metalloproteinases and other extracellular matrices contribute to maintenance of the vocal fold and development of pathology.
Authors: Ingo R Titze; Robert W Hitchcock; Kelly Broadhead; Ken Webb; Wenhua Li; Steven D Gray; Patrick A Tresco Journal: J Biomech Date: 2004-10 Impact factor: 2.712
Authors: Vladimir Vinarsky; Donald L Atkinson; Tamara J Stevenson; Mark T Keating; Shannon J Odelberg Journal: Dev Biol Date: 2005-03-01 Impact factor: 3.582
Authors: James A Cooper; LeeAnn O Bailey; Janell N Carter; Cynthia E Castiglioni; Michelle D Kofron; Frank K Ko; Cato T Laurencin Journal: Biomaterials Date: 2006-01-18 Impact factor: 12.479
Authors: Erik R Swanson; Tsunehisa Ohno; Dave Abdollahian; C Gaelyn Garrett; Bernard Rousseau Journal: Otolaryngol Head Neck Surg Date: 2010-10 Impact factor: 3.497
Authors: Erik R Swanson; Davood Abdollahian; Tsunehisa Ohno; Pingjiang Ge; David L Zealear; Bernard Rousseau Journal: Laryngoscope Date: 2009-07 Impact factor: 3.325
Authors: Bernard Rousseau; Ping Jiang Ge; Tsunehisa Ohno; Lesley C French; Susan L Thibeault Journal: Ann Otol Rhinol Laryngol Date: 2008-08 Impact factor: 1.547