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Literature DB >> 18022378

Adsorption and elution characteristics of nucleic acids on silica surfaces and their use in designing a miniaturized purification unit.

Tyson Poeckh¹, Santiago Lopez, A Oveta Fuller, Michael J Solomon, Ronald G Larson.

Abstract

We report nucleic acid (NA) adsorption isotherms and elution profiles for silica surfaces and use these to design a miniaturized NA purification unit based on solid-phase extraction with silica beads. The procedure is based on a pressure drop equation for flow through a packed bed and allows estimation of key design parameters such as channel dimensions, liquid flow rates, sample volume, and amount of silica needed. The usefulness of this design procedure is demonstrated by applying it to a column-based NA purification device for influenza detection for a case study of Madin-Darby canine kidney cells infected with influenza A virus.

Entities: Chemical Species

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Year: 2007 PMID： 18022378 PMCID： PMC2245989 DOI： 10.1016/j.ab.2007.10.026

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

10 in total

1. Integrated system for rapid PCR-based DNA analysis in microfluidic devices.

Authors: J Khandurina; T E McKnight; S C Jacobson; L C Waters; R S Foote; J M Ramsey
Journal: Anal Chem Date: 2000-07-01 Impact factor: 6.986

2. Evaluation of silica resins for direct and efficient extraction of DNA from complex biological matrices in a miniaturized format.

Authors: H Tian; A F Hühmer; J P Landers
Journal: Anal Biochem Date: 2000-08-01 Impact factor: 3.365

3. Toward a microchip-based solid-phase extraction method for isolation of nucleic acids.

Authors: Kelley A Wolfe; Michael C Breadmore; Jerome P Ferrance; Mary E Power; John F Conroy; Pamela M Norris; James P Landers
Journal: Electrophoresis Date: 2002-03 Impact factor: 3.535

4. Microchip-based purification of DNA from biological samples.

Authors: Michael C Breadmore; Kelley A Wolfe; Imee G Arcibal; Wayne K Leung; Dana Dickson; Braden C Giordano; Mary E Power; Jerome P Ferrance; Sanford H Feldman; Pamela M Norris; James P Landers
Journal: Anal Chem Date: 2003-04-15 Impact factor: 6.986

Review 5. Microfluidic devices fabricated in poly(dimethylsiloxane) for biological studies.

Authors: Samuel K Sia; George M Whitesides
Journal: Electrophoresis Date: 2003-11 Impact factor: 3.535

6. Polymerase chain reaction in high surface-to-volume ratio SiO2 microstructures.

Authors: Madhavi Krishnan; David T Burke; Mark A Burns
Journal: Anal Chem Date: 2004-11-15 Impact factor: 6.986

7. Rapid and simple method for purification of nucleic acids.

Authors: R Boom; C J Sol; M M Salimans; C L Jansen; P M Wertheim-van Dillen; J van der Noordaa
Journal: J Clin Microbiol Date: 1990-03 Impact factor: 5.948

8. Adsorption of organic molecules on silica surface.

Authors: Sudam K Parida; Sukalyan Dash; Sabita Patel; B K Mishra
Journal: Adv Colloid Interface Sci Date: 2006-07-31 Impact factor: 12.984

9. Chip PCR. II. Investigation of different PCR amplification systems in microbabricated silicon-glass chips.

Authors: J Cheng; M A Shoffner; G E Hvichia; L J Kricka; P Wilding
Journal: Nucleic Acids Res Date: 1996-01-15 Impact factor: 16.971

10. A fully integrated microfluidic genetic analysis system with sample-in-answer-out capability.

Authors: Christopher J Easley; James M Karlinsey; Joan M Bienvenue; Lindsay A Legendre; Michael G Roper; Sanford H Feldman; Molly A Hughes; Erik L Hewlett; Tod J Merkel; Jerome P Ferrance; James P Landers
Journal: Proc Natl Acad Sci U S A Date: 2006-12-11 Impact factor: 11.205

10 in total

Adsorption and elution characteristics of nucleic acids on silica surfaces and their use in designing a miniaturized purification unit.

1. Integrated system for rapid PCR-based DNA analysis in microfluidic devices.

2. Evaluation of silica resins for direct and efficient extraction of DNA from complex biological matrices in a miniaturized format.

3. Toward a microchip-based solid-phase extraction method for isolation of nucleic acids.

4. Microchip-based purification of DNA from biological samples.

Review 5. Microfluidic devices fabricated in poly(dimethylsiloxane) for biological studies.

6. Polymerase chain reaction in high surface-to-volume ratio SiO2 microstructures.

7. Rapid and simple method for purification of nucleic acids.

8. Adsorption of organic molecules on silica surface.

9. Chip PCR. II. Investigation of different PCR amplification systems in microbabricated silicon-glass chips.

10. A fully integrated microfluidic genetic analysis system with sample-in-answer-out capability.

1. Aptamer Recognition of Multiplexed Small-Molecule-Functionalized Substrates.

2. Adsorption and desorption of DNA-functionalized beads in glass microfluidic channels.

3. A Simple Thermoplastic Substrate Containing Hierarchical Silica Lamellae for High-Molecular-Weight DNA Extraction.

4. Fundamentals of magnet-actuated droplet manipulation on an open hydrophobic surface.

5. Sample preparation module for bacterial lysis and isolation of DNA from human urine.

6. DNA adsorption to and elution from silica surfaces: influence of amino acid buffers.

Review 7. Towards non- and minimally instrumented, microfluidics-based diagnostic devices.

8. A Rapid and Economical Method for Efficient DNA Extraction from Diverse Soils Suitable for Metagenomic Applications.

9. Low concentration DNA extraction and recovery using a silica solid phase.

10. Silicon dioxide thin film mediated single cell nucleic acid isolation.