Literature DB >> 17909288

Crystallization and preliminary crystallographic analysis of the ferredoxin component of carbazole 1,9a-dioxygenase from Nocardioides aromaticivorans IC177.

Kengo Inoue1, Yuji Ashikawa, Yusuke Usami, Haruko Noguchi, Zui Fujimoto, Hisakazu Yamane, Hideaki Nojiri.   

Abstract

Carbazole 1,9a-dioxygenase (CARDO) catalyzes the dihydroxylation of carbazole by angular position (C9a) carbon bonding to the imino nitrogen and its adjacent C1 carbon. CARDO consists of a terminal oxygenase component and two electron-transfer components: ferredoxin and ferredoxin reductase. The ferredoxin component of carbazole 1,9a-dioxygenase from Nocardioides aromaticivorans IC177 was crystallized at 293 K using the hanging-drop vapour-diffusion method with ammonium sulfate as the precipitant. The crystals, which were improved by macroseeding, diffract to 2.0 A resolution and belong to space group P4(1)2(1)2.

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Year:  2007        PMID： 17909288      PMCID： PMC2339720          DOI： 10.1107/S1744309107041437

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  19 in total

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3.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

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Review 4.  Bacterial metabolism of fluorene, dibenzofuran, dibenzothiophene, and carbazole.

Authors:  D C Bressler; P M Fedorak
Journal:  Can J Microbiol       Date:  2000-05       Impact factor: 2.419

5.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

6.  Identification and characterization of genes encoding carbazole 1,9a-dioxygenase in Pseudomonas sp. strain CA10.

Authors:  S I Sato; J W Nam; K Kasuga; H Nojiri; H Yamane; T Omori
Journal:  J Bacteriol       Date:  1997-08       Impact factor: 3.490

7.  Electron transfer complex formation between oxygenase and ferredoxin components in Rieske nonheme iron oxygenase system.

Authors:  Yuji Ashikawa; Zui Fujimoto; Haruko Noguchi; Hiroshi Habe; Toshio Omori; Hisakazu Yamane; Hideaki Nojiri
Journal:  Structure       Date:  2006-12       Impact factor: 5.006

Review 8.  Genetics of polycyclic aromatic hydrocarbon metabolism in diverse aerobic bacteria.

Authors:  Hiroshi Habe; Toshio Omori
Journal:  Biosci Biotechnol Biochem       Date:  2003-02       Impact factor: 2.043

9.  Divergent structures of carbazole degradative car operons isolated from gram-negative bacteria.

Authors:  Kengo Inoue; Jaka Widada; Seiichiro Nakai; Takayuki Endoh; Masaaki Urata; Yuji Ashikawa; Masaki Shintani; Yuko Saiki; Takako Yoshida; Hiroshi Habe; Toshio Omori; Hideaki Nojiri
Journal:  Biosci Biotechnol Biochem       Date:  2004-07       Impact factor: 2.043

Review 10.  Degradation of dioxin-like compounds by microorganisms.

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Journal:  Appl Microbiol Biotechnol       Date:  1998-05       Impact factor: 4.813
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  1 in total

1.  Crystallization and preliminary X-ray diffraction studies of a novel ferredoxin involved in the dioxygenation of carbazole by Novosphingobium sp. KA1.

Authors:  Takashi Umeda; Junichi Katsuki; Yusuke Usami; Kengo Inoue; Haruko Noguchi; Zui Fujimoto; Yuji Ashikawa; Hisakazu Yamane; Hideaki Nojiri
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2008-06-11
  1 in total

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