Literature DB >> 17884211

Commercial ampholytes used for isoelectric focusing may interfere with bioactivity based purification of antimicrobial peptides.

Shadi Riazi1, Sara Dover, Yevgeniy Turovskiy, Michael L Chikindas.   

Abstract

BioRad's Rotofor system has been frequently used for the purification of proteins and smaller peptides such as bacteriocins. In this study, we report that some commercially available ampholytes used with the Rotofor isoelectric focusing system possess antimicrobial activity, which may interfere with the purification of bacteriocins and bacteriocin-like substances.

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Year:  2007        PMID： 17884211      PMCID： PMC2040054          DOI： 10.1016/j.mimet.2007.08.002

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  9 in total

Review 1.  Bacteriocins: safe, natural antimicrobials for food preservation.

Authors:  J Cleveland; T J Montville; I F Nes; M L Chikindas
Journal:  Int J Food Microbiol       Date:  2001-12-04       Impact factor: 5.277

2.  Conductivity properties of carrier ampholyte pH gradients in isoelectric focusing.

Authors:  Alexander V Stoyanov; Champak Das; Carl K Fredrickson; Z Hugh Fan
Journal:  Electrophoresis       Date:  2005-01       Impact factor: 3.535

3.  Rapid and Efficient Purification Method for Small, Hydrophobic, Cationic Bacteriocins: Purification of Lactococcin B and Pediocin PA-1.

Authors:  K Venema; M L Chikindas; J Seegers; A J Haandrikman; K J Leenhouts; G Venema; J Kok
Journal:  Appl Environ Microbiol       Date:  1997-01       Impact factor: 4.792

4.  Use of Rotofor preparative isoelectrofocusing cell in protein purification procedure.

Authors:  A Ayala; J Parrado; A Machado
Journal:  Appl Biochem Biotechnol       Date:  1998-01       Impact factor: 2.926

5.  Not only the nature of peptide but also the characteristics of cell membrane determine the antimicrobial mechanism of a peptide.

Authors:  J F Liang; S C Kim
Journal:  J Pept Res       Date:  1999-05

Review 6.  Bacteriocins: modes of action and potentials in food preservation and control of food poisoning.

Authors:  T Abee; L Krockel; C Hill
Journal:  Int J Food Microbiol       Date:  1995-12       Impact factor: 5.277

Review 7.  epsilon-Poly-L-lysine: microbial production, biodegradation and application potential.

Authors:  T Yoshida; T Nagasawa
Journal:  Appl Microbiol Biotechnol       Date:  2003-05-01       Impact factor: 4.813

8.  Isolation and purification of propionicin PLG-1, a bacteriocin produced by a strain of Propionibacterium thoenii.

Authors:  W J Lyon; B A Glatz
Journal:  Appl Environ Microbiol       Date:  1993-01       Impact factor: 4.792

9.  Pediocin PD-1, a bactericidal antimicrobial peptide from Pediococcus damnosus NCFB 1832.

Authors:  G Green; L M Dicks; G Bruggeman; E J Vandamme; M L Chikindas
Journal:  J Appl Microbiol       Date:  1997-07       Impact factor: 3.772
  9 in total
  2 in total

1.  Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method.

Authors:  Raja Veerapandian; Anuja Paudyal; Adeline Chang; Govindsamy Vediyappan
Journal:  J Vis Exp       Date:  2020-06-14       Impact factor: 1.355

2.  Mode of action and safety of lactosporin, a novel antimicrobial protein produced by Bacillus coagulans ATCC 7050.

Authors:  S Riazi; S E Dover; M L Chikindas
Journal:  J Appl Microbiol       Date:  2012-07-24       Impact factor: 3.772
  2 in total

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