Literature DB >> 16535495

Rapid and Efficient Purification Method for Small, Hydrophobic, Cationic Bacteriocins: Purification of Lactococcin B and Pediocin PA-1.

K Venema, M L Chikindas, J Seegers, A J Haandrikman, K J Leenhouts, G Venema, J Kok.   

Abstract

The bacteriocins lactococcin B and pediocin PA-1 were purified by ethanol precipitation, preparative isoelectric focusing, and ultrafiltration. The procedure reproducibly leads to high final yields in comparison to the generally low yields obtained by column chromatography. Specifically, during isoelectric focusing no loss of activity occurs. The method, in general, should be applicable to small, hydrophobic, cationic bacteriocins.

Entities:  

Year:  1997        PMID： 16535495      PMCID： PMC1389109          DOI： 10.1128/aem.63.1.305-309.1997

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  16 in total

1.  Purification and primary structure of pediocin PA-1 produced by Pediococcus acidilactici PAC-1.0.

Authors:  J T Henderson; A L Chopko; P D van Wassenaar
Journal:  Arch Biochem Biophys       Date:  1992-05-15       Impact factor: 4.013

2.  Cloning, sequencing, and expression in Escherichia coli of lcnB, a third bacteriocin determinant from the lactococcal bacteriocin plasmid p9B4-6.

Authors:  M J van Belkum; J Kok; G Venema
Journal:  Appl Environ Microbiol       Date:  1992-02       Impact factor: 4.792

3.  THE INHIBITING EFFECT OF STREPTOCOCCUS LACTIS ON LACTOBACILLUS BULGARICUS.

Authors:  L A Rogers
Journal:  J Bacteriol       Date:  1928-11       Impact factor: 3.490

4.  Potential of lactic streptococci to produce bacteriocin.

Authors:  A Geis; J Singh; M Teuber
Journal:  Appl Environ Microbiol       Date:  1983-01       Impact factor: 4.792

5.  Mutational analysis and chemical modification of Cys24 of lactococcin B, a bacteriocin produced by Lactococcus lactis.

Authors:  K Venema; M H Dost; G Venema; J Kok
Journal:  Microbiology       Date:  1996-10       Impact factor: 2.777

6.  Improved medium for lactic streptococci and their bacteriophages.

Authors:  B E Terzaghi; W E Sandine
Journal:  Appl Microbiol       Date:  1975-06

7.  Lactococcus lactis: high-level expression of tetanus toxin fragment C and protection against lethal challenge.

Authors:  J M Wells; P W Wilson; P M Norton; M J Gasson; R W Le Page
Journal:  Mol Microbiol       Date:  1993-06       Impact factor: 3.501

8.  Cloning and nucleotide base sequence analysis of a spectinomycin adenyltransferase AAD(9) determinant from Enterococcus faecalis.

Authors:  D J LeBlanc; L N Lee; J M Inamine
Journal:  Antimicrob Agents Chemother       Date:  1991-09       Impact factor: 5.191

9.  Lactococcin A, a new bacteriocin from Lactococcus lactis subsp. cremoris: isolation and characterization of the protein and its gene.

Authors:  H Holo; O Nilssen; I F Nes
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

10.  The leader peptide of colicin V shares consensus sequences with leader peptides that are common among peptide bacteriocins produced by gram-positive bacteria.

Authors:  L S Håvarstein; H Holo; I F Nes
Journal:  Microbiology (Reading)       Date:  1994-09       Impact factor: 2.777
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  6 in total

1.  Method for rapid purification of class IIa bacteriocins and comparison of their activities.

Authors:  D Guyonnet; C Fremaux; Y Cenatiempo; J M Berjeaud
Journal:  Appl Environ Microbiol       Date:  2000-04       Impact factor: 4.792

2.  Commercial ampholytes used for isoelectric focusing may interfere with bioactivity based purification of antimicrobial peptides.

Authors:  Shadi Riazi; Sara Dover; Yevgeniy Turovskiy; Michael L Chikindas
Journal:  J Microbiol Methods       Date:  2007-08-15       Impact factor: 2.363

Review 3.  Bacteriocin production and different strategies for their recovery and purification.

Authors:  Anita Kumari Garsa; Rashmi Kumariya; S K Sood; Anil Kumar; Suman Kapila
Journal:  Probiotics Antimicrob Proteins       Date:  2014-03       Impact factor: 4.609

Review 4.  Bacteriocins from lactic acid bacteria and their potential clinical applications.

Authors:  Abigail Fernandes; Renitta Jobby
Journal:  Appl Biochem Biotechnol       Date:  2022-03-15       Impact factor: 3.094

5.  Novel expression system for large-scale production and purification of recombinant class IIa bacteriocins and its application to piscicolin 126.

Authors:  Gerard M Gibbs; Barrie E Davidson; Alan J Hillier
Journal:  Appl Environ Microbiol       Date:  2004-06       Impact factor: 4.792

6.  Proteinase PrtP impairs lactococcin LcnB activity in Lactococcus lactis BGMN1-501: new insights into bacteriocin regulation.

Authors:  Goran Vukotic; Nemanja Mirkovic; Branko Jovcic; Marija Miljkovic; Ivana Strahinic; Djordje Fira; Zorica Radulovic; Milan Kojic
Journal:  Front Microbiol       Date:  2015-02-10       Impact factor: 5.640
  6 in total

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