Literature DB >> 9457755

Use of Rotofor preparative isoelectrofocusing cell in protein purification procedure.

A Ayala1, J Parrado, A Machado.   

Abstract

The Rotofor cell is a preparative isoelectric focusing (IEF) apparatus, in which IEF is performed entirely in free solution. Electrofocusing in Rotofor cell has been described as well-suited for use at any stage of a purification scheme. However, it has some important limitations in resolving complex mixtures of proteins. This paper describes the advantages and disadvantages of using the Rotofor cell in purification protocols.

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Year:  1998        PMID： 9457755     DOI： 10.1007/bf02786017

Source DB:  PubMed          Journal:  Appl Biochem Biotechnol        ISSN： 0273-2289            Impact factor:   2.926


  6 in total

1.  Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method.

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2.  Isolation of a distinct Mycobacterium tuberculosis mannose-capped lipoarabinomannan isoform responsible for recognition by CD1b-restricted T cells.

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Journal:  Glycobiology       Date:  2012-04-25       Impact factor: 4.313

3.  Commercial ampholytes used for isoelectric focusing may interfere with bioactivity based purification of antimicrobial peptides.

Authors:  Shadi Riazi; Sara Dover; Yevgeniy Turovskiy; Michael L Chikindas
Journal:  J Microbiol Methods       Date:  2007-08-15       Impact factor: 2.363

4.  Influence of secreted factors from human adipose tissue on glucose utilization and proinflammatory reaction.

Authors:  Karine Tréguer; Rodolphe Dusaulcy; Sandra Grès; Estelle Wanecq; Philippe Valet; Jean Sébastien Saulnier-Blache
Journal:  J Physiol Biochem       Date:  2013-01-27       Impact factor: 4.158

Review 5.  Top Down proteomics: facts and perspectives.

Authors:  Adam D Catherman; Owen S Skinner; Neil L Kelleher
Journal:  Biochem Biophys Res Commun       Date:  2014-02-17       Impact factor: 3.575

Review 6.  A Comprehensive Guide for Performing Sample Preparation and Top-Down Protein Analysis.

Authors:  Matthew P Padula; Iain J Berry; Matthew B O Rourke; Benjamin B A Raymond; Jerran Santos; Steven P Djordjevic
Journal:  Proteomes       Date:  2017-04-07
  6 in total

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