Literature DB >> 17868768

PLP catabolism: identification of the 4-pyridoxic acid dehydrogenase gene in Mesorhizobium loti MAFF303099.

Tathagata Mukherjee1, Cynthia Kinsland, Tadhg P Begley.   

Abstract

The function of the mlr6793 gene from Mesorhizobium loti MAFF303099 has been identified. This gene encodes 4-pyridoxic acid dehydrogenase, an enzyme involved in the catabolism of PLP (Vitamin B6). This enzyme was overexpressed in Escherichia coli and characterized. 4-Pyridoxic acid dehydrogenase is a 33kDa protein that catalyzes the four electron oxidation of 4-pyridoxic acid to 3-hydroxy-2-methylpyridine-4,5-dicarboxylate, using nicotinamide adenine dinucleotide as a cofactor. The k cat for NADH production is 0.01s(-1). The KM values for 4-pyridoxic acid and NAD are 5.8 and 6.6microM, respectively.

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Year:  2007        PMID： 17868768      PMCID： PMC2140245          DOI： 10.1016/j.bioorg.2007.07.003

Source DB:  PubMed          Journal:  Bioorg Chem        ISSN： 0045-2068            Impact factor:   5.275


  6 in total

1.  Bacterial oxidation of vitamin B6. II. Metabolites of pyridoxamine.

Authors:  R W BURG; V W RODWELL; E E SNELL
Journal:  J Biol Chem       Date:  1960-04       Impact factor: 5.157

2.  4-Pyridoxolactonase from a symbiotic nitrogen-fixing bacterium Mesorhizobium loti: cloning, expression, and characterization.

Authors:  Junichi Funami; Yu Yoshikane; Hitoshi Kobayashi; Nana Yokochi; Baiqiang Yuan; Kozo Iwasaki; Kouhei Ohnishi; Toshiharu Yagi
Journal:  Biochim Biophys Acta       Date:  2005-10-03

3.  Identification of a new tetrameric pyridoxal 4-dehydrogenase as the second enzyme in the degradation pathway for pyridoxine in a nitrogen-fixing symbiotic bacterium, Mesorhizobium loti.

Authors:  Nana Yokochi; Saki Nishimura; Yu Yoshikane; Kouhei Ohnishi; Toshiharu Yagi
Journal:  Arch Biochem Biophys       Date:  2006-06-16       Impact factor: 4.013

4.  The nitrogen-fixing symbiotic bacterium Mesorhizobium loti has and expresses the gene encoding pyridoxine 4-oxidase involved in the degradation of vitamin B6.

Authors:  Baiqiang Yuan; Yu Yoshikane; Nana Yokochi; Kouhei Ohnishi; Toshiharu Yagi
Journal:  FEMS Microbiol Lett       Date:  2004-05-15       Impact factor: 2.742

5.  The bacterial oxidation of vitamin B6. 4-Pyridoxic acid dehydrogenase: a membrane-bound enzyme from Pseudomonas MA-1.

Authors:  T Yagi; G M Kishore; E E Snell
Journal:  J Biol Chem       Date:  1983-08-10       Impact factor: 5.157

6.  Use of 8-substituted-FAD analogues to investigate the hydroxylation mechanism of the flavoprotein 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase.

Authors:  Pimchai Chaiyen; Jeerus Sucharitakul; Jisnuson Svasti; Barrie Entsch; Vincent Massey; David P Ballou
Journal:  Biochemistry       Date:  2004-04-06       Impact factor: 3.162
  6 in total
  5 in total

1.  Structure determination and characterization of the vitamin B6 degradative enzyme (E)-2-(acetamidomethylene)succinate hydrolase.

Authors:  Kathryn M McCulloch; Tathagata Mukherjee; Tadhg P Begley; Steven E Ealick
Journal:  Biochemistry       Date:  2010-02-16       Impact factor: 3.162

2.  Structure of the PLP degradative enzyme 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase from Mesorhizobium loti MAFF303099 and its mechanistic implications.

Authors:  Kathryn M McCulloch; Tathagata Mukherjee; Tadhg P Begley; Steven E Ealick
Journal:  Biochemistry       Date:  2009-05-19       Impact factor: 3.162

3.  Gene identification and structural characterization of the pyridoxal 5'-phosphate degradative protein 3-hydroxy-2-methylpyridine-4,5-dicarboxylate decarboxylase from mesorhizobium loti MAFF303099.

Authors:  Tathagata Mukherjee; Kathryn M McCulloch; Steven E Ealick; Tadhg P Begley
Journal:  Biochemistry       Date:  2007-10-31       Impact factor: 3.162

Review 4.  Vitamin B6: a long known compound of surprising complexity.

Authors:  Sutton Mooney; Jan-Erik Leuendorf; Christopher Hendrickson; Hanjo Hellmann
Journal:  Molecules       Date:  2009-01-12       Impact factor: 4.411

5.  How does (E)-2-(acetamidomethylene)succinate bind to its hydrolase? From the binding process to the final result.

Authors:  Ji-Long Zhang; Qing-Chuan Zheng; Zheng-Qiang Li; Hong-Xing Zhang
Journal:  PLoS One       Date:  2013-01-07       Impact factor: 3.240
  5 in total

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