BACKGROUND: A new portable confocal laser microscopy system has recently been developed. OBJECTIVE: Our purpose was to evaluate the feasibility of performing real-time microscopic imaging with a prototype of a new high-resolution miniprobe in conjunction with a video mosaicing algorithm. DESIGN: Feasibility study. SETTING: Tertiary referral center at a large university hospital. PATIENTS: Seven patients referred for endoscopy for various indications. MAIN OUTCOME MEASUREMENTS: High-resolution laser microscopy of the upper and lower GI tract was performed with standard endoscopes. Seven to 10 mL of 1% fluorescein was injected intravenously a few seconds before the procedure. No additional preparation was required. The prototype used has a lateral resolution of 1.2 microm and an axial resolution of 3 mum with a total field of 240 x 200 microm. From all stored video sequences a video mosaicing algorithm was used to combine the successive individual images, cancel motion artifacts, and reconstitute panoramas of the tissues. RESULTS: Cell-to-cell borders, single cell structures, and mucosal inflammation was readily detectable. By the use of the mosaicing algorithm, the image area could be increased 2- to 4-fold, and image definition could be further enhanced to allow finer detail visualization. LIMITATIONS: Low number of patients, early feasibility study. CONCLUSIONS: Our preliminary data show that high-resolution miniprobe-based confocal fluorescence microscopy in conjunction with video mosaicing has the potential to provide images similar to standard histopathologic studies. Dynamic images with a smaller field of view can be combined to reconstruct still images of high resolution covering a fairly large area.
BACKGROUND: A new portable confocal laser microscopy system has recently been developed. OBJECTIVE: Our purpose was to evaluate the feasibility of performing real-time microscopic imaging with a prototype of a new high-resolution miniprobe in conjunction with a video mosaicing algorithm. DESIGN: Feasibility study. SETTING: Tertiary referral center at a large university hospital. PATIENTS: Seven patients referred for endoscopy for various indications. MAIN OUTCOME MEASUREMENTS: High-resolution laser microscopy of the upper and lower GI tract was performed with standard endoscopes. Seven to 10 mL of 1% fluorescein was injected intravenously a few seconds before the procedure. No additional preparation was required. The prototype used has a lateral resolution of 1.2 microm and an axial resolution of 3 mum with a total field of 240 x 200 microm. From all stored video sequences a video mosaicing algorithm was used to combine the successive individual images, cancel motion artifacts, and reconstitute panoramas of the tissues. RESULTS: Cell-to-cell borders, single cell structures, and mucosal inflammation was readily detectable. By the use of the mosaicing algorithm, the image area could be increased 2- to 4-fold, and image definition could be further enhanced to allow finer detail visualization. LIMITATIONS: Low number of patients, early feasibility study. CONCLUSIONS: Our preliminary data show that high-resolution miniprobe-based confocal fluorescence microscopy in conjunction with video mosaicing has the potential to provide images similar to standard histopathologic studies. Dynamic images with a smaller field of view can be combined to reconstruct still images of high resolution covering a fairly large area.
Authors: Richard C Newton; David P Noonan; Valentina Vitiello; James Clark; Christopher J Payne; Jianzhong Shang; Mikael Sodergren; Ara Darzi; Guang-Zhong Yang Journal: Surg Endosc Date: 2012-04-26 Impact factor: 4.584
Authors: Simon C Schlachter; Dongkyun Kang; Michalina J Gora; Paulino Vacas-Jacques; Tao Wu; Robert W Carruth; Eric J Wilsterman; Brett E Bouma; Kevin Woods; Guillermo J Tearney Journal: Biomed Opt Express Date: 2013-08-13 Impact factor: 3.732
Authors: Dongkyun Kang; Robert W Carruth; Minkyu Kim; Simon C Schlachter; Milen Shishkov; Kevin Woods; Nima Tabatabaei; Tao Wu; Guillermo J Tearney Journal: Biomed Opt Express Date: 2013-09-03 Impact factor: 3.732