PURPOSE: To investigate the clinical application of fluorescence in situ hybridization (FISH) for assessing chromosome disorders of embryos in preimplantation diagnosis of carriers with der(15)t(Y;15)(q12;p11) translocations. METHODS: Multicolor FISH was performed using directly-labelled DNA probes, chromosome X with one (DXZ1, Xp11.1-q11.1), but Y with two (DYZ3, Yp11.1-q11.1 and DYZ1, Yq12). Normal embryos were transferred on day 6 at blastocyst stage. RESULTS: Couple A: Three of 6 biopsied embryos were normal. Two normal blastocysts were transferred, but no pregnancy was achieved. Couple B: Three of 6 biopsied embryos were normal. Two normal blastocysts were transferred. A normal male infant weighing 3,230 g was born by cesarean section on the 39th week of gestation. All of the remaining nonreplaced embryos showed mosaic or der(15). CONCLUSION: Embryos from carries of der(15)t(Y;15)(q12;p11) translocation showed a high frequency of chromosome abnormalities. PGD is a valuable screen tool for those couples to treat their infertility and break the transmission of der(15) chromosome for their offspring.
PURPOSE: To investigate the clinical application of fluorescence in situ hybridization (FISH) for assessing chromosome disorders of embryos in preimplantation diagnosis of carriers with der(15)t(Y;15)(q12;p11) translocations. METHODS: Multicolor FISH was performed using directly-labelled DNA probes, chromosome X with one (DXZ1, Xp11.1-q11.1), but Y with two (DYZ3, Yp11.1-q11.1 and DYZ1, Yq12). Normal embryos were transferred on day 6 at blastocyst stage. RESULTS: Couple A: Three of 6 biopsied embryos were normal. Two normal blastocysts were transferred, but no pregnancy was achieved. Couple B: Three of 6 biopsied embryos were normal. Two normal blastocysts were transferred. A normal male infant weighing 3,230 g was born by cesarean section on the 39th week of gestation. All of the remaining nonreplaced embryos showed mosaic or der(15). CONCLUSION: Embryos from carries of der(15)t(Y;15)(q12;p11) translocation showed a high frequency of chromosome abnormalities. PGD is a valuable screen tool for those couples to treat their infertility and break the transmission of der(15) chromosome for their offspring.
Authors: E Iwarsson; H Malmgren; J Inzunza; L Ahrlund-Richter; P Sjöblom; B Rosenlund; M Fridström; O Hovatta; M Nordenskjöld; E Blennow Journal: Prenat Diagn Date: 2000-12 Impact factor: 3.050
Authors: Chun Kyu Lim; Jin Hyun Jun; Dong Mi Min; Hyoung-Song Lee; Jin Young Kim; Mi Kyoung Koong; Inn Soo Kang Journal: Prenat Diagn Date: 2004-07 Impact factor: 3.050
Authors: Maria Bucksch; Monika Ziegler; Nadezda Kosayakova; Milene V Mulatinho; Milene V Mulhatino; Juan C Llerena; Susanne Morlot; Wolfgang Fischer; Anna D Polityko; Anna I Kulpanovich; Michael B Petersen; Britta Belitz; Vladimir Trifonov; Anja Weise; Thomas Liehr; Ahmed B Hamid Journal: J Histochem Cytochem Date: 2012-04-17 Impact factor: 2.479