D I Dozortsev1, K T McGinnis. 1. Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Hutzel Hospital, Wayne State University, Detroit, MI 48201, USA. aa4421@wayne.edu
Abstract
OBJECTIVE: To improve existing preimplantation genetic diagnosis fixation techniques. DESIGN: Prospective randomized in vitro study. SETTING: Academic medical center. PATIENT(S): None. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): The intensity and clarity of fluorescence in situ hybridization (FISH) signals and the percentage of successfully fixed blastomeres. RESULT(S): The described fixation technique resulted in 100% fixation and 100% adequate FISH signals. Two conventional techniques resulted in 94% and 87% fixation, and in adequate FISH signals in 81% and 87%, respectively. CONCLUSION(S): This newly developed fixation technique simplifies the process of fixation of blastomeres for preimplantation diagnosis while essentially eliminating the possibility of losing a cell during fixation. It will hopefully allow more IVF programs to offer their patients preimplantation genetic diagnosis using the FISH technique.
OBJECTIVE: To improve existing preimplantation genetic diagnosis fixation techniques. DESIGN: Prospective randomized in vitro study. SETTING: Academic medical center. PATIENT(S): None. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): The intensity and clarity of fluorescence in situ hybridization (FISH) signals and the percentage of successfully fixed blastomeres. RESULT(S): The described fixation technique resulted in 100% fixation and 100% adequate FISH signals. Two conventional techniques resulted in 94% and 87% fixation, and in adequate FISH signals in 81% and 87%, respectively. CONCLUSION(S): This newly developed fixation technique simplifies the process of fixation of blastomeres for preimplantation diagnosis while essentially eliminating the possibility of losing a cell during fixation. It will hopefully allow more IVF programs to offer their patients preimplantation genetic diagnosis using the FISH technique.
Authors: Raquel M Fernández; Ana Peciña; Beatriz Sánchez; Maria Dolores Lozano-Arana; Juan Carlos García-Lozano; Rosario Pérez-Garrido; Ramiro Núñez; Salud Borrego; Guillermo Antiñolo Journal: Biomed Res Int Date: 2015-07-16 Impact factor: 3.411