Literature DB >> 17706197

Deletion of the MTO2 gene related to tRNA modification causes a failure in mitochondrial RNA metabolism in the yeast Saccharomyces cerevisiae.

Xinjian Wang1, Qingfeng Yan, Min-Xin Guan.   

Abstract

We report here the characterization of the yeast mto2 null mutants carrying wild-type mitochondrial DNA or 15S rRNA C1049G allele. The amounts of mitochondrial tRNA(Lys), tRNA(Glu), tRNA(Gln), tRNA(Leu), tRNA(Gly) and tRNA(Met) were markedly decreased but those of tRNA(Arg) and tRNA(His) were not affected in mto2 strains. The mto2 strains exhibited significant reduction in the aminoacylation of tRNA(Lys), tRNA(Leu) but almost no effect in those of tRNA(His). Interestingly, the strain carrying the C1049G allele exhibited an impairment of aminoacylation of those tRNAs. Furthermore, the steady-state levels of mitochondrial mRNA CYTB, COX1, COX2, COX3, and ATP6 were markedly decreased in mto2 strains. These data strongly indicate that unmodified tRNA caused by the deletion of MTO2 caused the instability of mitochondrial tRNAs and mRNAs and impairment of aminoacylation of tRNAs.

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Year:  2007        PMID： 17706197     DOI： 10.1016/j.febslet.2007.07.067

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  9 in total

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4.  Combination of the loss of cmnm5U34 with the lack of s2U34 modifications of tRNALys, tRNAGlu, and tRNAGln altered mitochondrial biogenesis and respiration.

Authors:  Xinjian Wang; Qingfeng Yan; Min-Xin Guan
Journal:  J Mol Biol       Date:  2009-12-11       Impact factor: 5.469

5.  Mitochondrial tRNAThr G15927A mutation may modulate the phenotypic manifestation of ototoxic 12S rRNA A1555G mutation in four Chinese families.

Authors:  Xinjian Wang; Jianxin Lu; Yi Zhu; Aifen Yang; Li Yang; Ronghua Li; Bobei Chen; Yaping Qian; Xiaowen Tang; Jindan Wang; Xue Zhang; Min-Xin Guan
Journal:  Pharmacogenet Genomics       Date:  2008-12       Impact factor: 2.089

6.  Mutation in MTO1 involved in tRNA modification impairs mitochondrial RNA metabolism in the yeast Saccharomyces cerevisiae.

Authors:  Xinjian Wang; Qingfeng Yan; Min-Xin Guan
Journal:  Mitochondrion       Date:  2009-01-30       Impact factor: 4.160

7.  MTO1 worked as a modifier in the aminoglycosides sensitivity of yeast carrying a mitochondrial 15S rRNA C1477G mutation.

Authors:  Xiaoyu Zhu; Xiangyu He; Wei Wang; Qiyin Zhou; Zhe Yu; Yu Dai; Xufen Zhu; Qingfeng Yan
Journal:  PLoS One       Date:  2015-04-21       Impact factor: 3.240

8.  Nuclear modifier MTO2 modulates the aminoglycoside-sensitivity of mitochondrial 15S rRNA C1477G mutation in Saccharomyces cerevisiae.

Authors:  Xiangyu He; Xiaoyu Zhu; Xuexiang Wang; Wei Wang; Yu Dai; Qingfeng Yan
Journal:  PLoS One       Date:  2013-12-10       Impact factor: 3.240

9.  Mechanistic study on the nuclear modifier gene MSS1 mutation suppressing neomycin sensitivity of the mitochondrial 15S rRNA C1477G mutation in Saccharomyces cerevisiae.

Authors:  Qiyin Zhou; Wei Wang; Xiangyu He; Xiaoyu Zhu; Yaoyao Shen; Zhe Yu; Xuexiang Wang; Xuchen Qi; Xuan Zhang; Mingjie Fan; Yu Dai; Shuxu Yang; Qingfeng Yan
Journal:  PLoS One       Date:  2014-03-03       Impact factor: 3.240
  9 in total

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