OBJECTIVE: To investigate the regulatory effects of nerve growth factor (NGF) on basal and capsaicin-induced release of neuropeptide substance P (SP) in primary cultured embryonic rat dorsal root ganglion (DRG) neurons. METHODS: DRGs were dissected from 15-day-old embryonic Wistar rats. DRG neurons were dissociated and cultured, and then exposed to different concentrations of NGF (10 ng/mL, 30 ng/mL, or 100 ng/mL) for 72 h. The neurons cultured in media without NGF served as control. RT-PCR were used for detecting the mRNAs of SP and vanilloid receptor 1 (VR1) in the DRG neurons. The SP basal and capsaicin (100 nmol/L)-induced release in the culture were measured by radioimmunoassay (RIA). RESULTS: SP mRNA and VR1 mRNA expression increased in primary cultured DRG neurons in a dose-dependent manner of NGF. Both basal release and capsaicin-evoked release of SP increased in NGF-treated DRG neurons compared with in control group. The capsaicin-evoked release of SP also increased in a dose-dependent manner of NGF. CONCLUSION: NGF may promote both basal release and capsaicin-evoked release of SP. NGF might increase the sensitivity of nociceptors by increasing the SP mRNA or VR1 mRNA.
OBJECTIVE: To investigate the regulatory effects of nerve growth factor (NGF) on basal and capsaicin-induced release of neuropeptide substance P (SP) in primary cultured embryonic rat dorsal root ganglion (DRG) neurons. METHODS: DRGs were dissected from 15-day-old embryonic Wistar rats. DRG neurons were dissociated and cultured, and then exposed to different concentrations of NGF (10 ng/mL, 30 ng/mL, or 100 ng/mL) for 72 h. The neurons cultured in media without NGF served as control. RT-PCR were used for detecting the mRNAs of SP and vanilloid receptor 1 (VR1) in the DRG neurons. The SP basal and capsaicin (100 nmol/L)-induced release in the culture were measured by radioimmunoassay (RIA). RESULTS: SP mRNA and VR1 mRNA expression increased in primary cultured DRG neurons in a dose-dependent manner of NGF. Both basal release and capsaicin-evoked release of SP increased in NGF-treated DRG neurons compared with in control group. The capsaicin-evoked release of SP also increased in a dose-dependent manner of NGF. CONCLUSION:NGF may promote both basal release and capsaicin-evoked release of SP. NGF might increase the sensitivity of nociceptors by increasing the SP mRNA or VR1 mRNA.
Authors: Adalberto Merighi; Giorgio Carmignoto; Sara Gobbo; Laura Lossi; Chiara Salio; Angela Maria Vergnano; Michaela Zonta Journal: Prog Brain Res Date: 2004 Impact factor: 2.453
Authors: Theodore J Price; Michael D Louria; Damaries Candelario-Soto; Gregory O Dussor; Nathanial A Jeske; Amol M Patwardhan; Anibal Diogenes; Amanda A Trott; Kenneth M Hargreaves; Christopher M Flores Journal: BMC Neurosci Date: 2005-01-24 Impact factor: 3.288
Authors: Kim Newberry; Shuya Wang; Nina Hoque; Laszlo Kiss; Michael K Ahlijanian; James Herrington; John D Graef Journal: J Neurophysiol Date: 2016-04-06 Impact factor: 2.714
Authors: Jan L Bjersing; Mats Dehlin; Malin Erlandsson; Maria I Bokarewa; Kaisa Mannerkorpi Journal: Arthritis Res Ther Date: 2012-07-09 Impact factor: 5.156