Walter G Besio1, Kanthaiah Koka, Andrew J Cole. 1. Department of Biomedical Engineering, Louisiana Tech University, Ruston, Louisiana 71272, USA. walterb@latech.edu
Abstract
PURPOSE: The aim of this pilot study was to investigate the antiepileptic effects of a novel noninvasive stimulation technique, transcutaneous electrical stimulation (TcES) via scalp concentric ring electrodes, on pilocarpine-induced status epilepticus (SE) in rats. METHODS: Five minutes after the onset of SE, TcES was administered to the experimental rat via bipolar concentric ring electrode at the CZ location. Symmetrical, biphasic, charge-balanced, constant current, isolated pulses were applied via a custom-made stimulator. TcES parameters ranged from 200-750 Hz, 200 or 300 mus pulse duration, and 50 or 60 mA, applied for 1 min, started with the least intense parameter set and progressively increased. RESULTS: TcES attenuated electrographic seizure activity and halted the progression of behavioral seizures. Interruption of seizure activity outlasted the period of stimulation and appeared to be long-lasting. TcES treatment significantly extended the life and enhanced the survival of rats after SE. CONCLUSIONS: Noninvasive TcES, applied 5 min after SE onset via novel concentric ring electrodes on the scalp, reduced, or abolished electrographic and behavioral seizure activity in pilocarpine-induced SE in rats. These findings suggest that TcES may have a role in the treatment of SE.
PURPOSE: The aim of this pilot study was to investigate the antiepileptic effects of a novel noninvasive stimulation technique, transcutaneous electrical stimulation (TcES) via scalp concentric ring electrodes, on pilocarpine-induced status epilepticus (SE) in rats. METHODS: Five minutes after the onset of SE, TcES was administered to the experimental rat via bipolar concentric ring electrode at the CZ location. Symmetrical, biphasic, charge-balanced, constant current, isolated pulses were applied via a custom-made stimulator. TcES parameters ranged from 200-750 Hz, 200 or 300 mus pulse duration, and 50 or 60 mA, applied for 1 min, started with the least intense parameter set and progressively increased. RESULTS: TcES attenuated electrographic seizure activity and halted the progression of behavioral seizures. Interruption of seizure activity outlasted the period of stimulation and appeared to be long-lasting. TcES treatment significantly extended the life and enhanced the survival of rats after SE. CONCLUSIONS: Noninvasive TcES, applied 5 min after SE onset via novel concentric ring electrodes on the scalp, reduced, or abolished electrographic and behavioral seizure activity in pilocarpine-induced SE in rats. These findings suggest that TcES may have a role in the treatment of SE.
Authors: Oleksandr Makeyev; Xiang Liu; Hiram Luna-Munguía; Gabriela Rogel-Salazar; Samuel Mucio-Ramirez; Yuhong Liu; Yan L Sun; Steven M Kay; Walter G Besio Journal: IEEE Trans Neural Syst Rehabil Eng Date: 2012-07 Impact factor: 3.802
Authors: Walter G Besio; Iris E Martínez-Juárez; Oleksandr Makeyev; John N Gaitanis; Andrew S Blum; Robert S Fisher; Andrei V Medvedev Journal: IEEE J Transl Eng Health Med Date: 2014-06-30 Impact factor: 3.316