| Literature DB >> 17625166 |
Fred A Rainey1, Margarida Ferreira2, M Fernanda Nobre3, Keren Ray1, Danielle Bagaley1, Ashlee M Earl1, John R Battista1, Benito Gómez-Silva4, Christopher P McKay5, Milton S da Costa2.
Abstract
Three ionizing-radiation-resistant bacterial strains (designated KR-196, KR-198 and KR-200(T)) were isolated from a sample of arid soil collected from a coastal desert in Chile. The soil sample was irradiated before serial dilution plating was performed using one-tenth-strength plate count agar. Phylogenetic analysis of the 16S rRNA gene sequences showed these organisms to represent a novel species of the genus Deinococcus, having sequence similarities of 87.3-90.8 % with respect to recognized Deinococcus species. Strains KR-196, KR-198 and KR-200(T) were aerobic and showed optimum growth at 30 degrees C and pH 6.5-8.0. The major respiratory menaquinone was MK-8. The predominant fatty acids in these strains were 16 : 1 omega 7c, 16 : 0, 15 : 1 omega 6c, 17 : 0 and 18 : 0. The DNA G+C content of strain KR-200(T) was 63.9 mol%. Strains KR-196, KR-198 and KR-200(T) were found to be resistant to >10 kGy gamma radiation. On the basis of the phylogenetic, chemotaxonomic and phenotypic data, strain KR-200(T) represents a novel species of the genus Deinococcus, for which the name Deinococcus peraridilitoris sp. nov. is proposed. The type strain is KR-200(T) (=LMG 22246(T)=CIP 109416(T)).Entities:
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Year: 2007 PMID： 17625166 DOI： 10.1099/ijs.0.64956-0
Source DB: PubMed Journal: Int J Syst Evol Microbiol ISSN： 1466-5026 Impact factor: 2.747