AIM: To evaluate chromosome aberration and fluorescent in situ hybridization (FISH) assays as a method to estimate of health risk, we monitored 9 male subjects occupationally exposed to low doses of both ionizing radiation and ultrasound during a period of over 3 years. METHODS: Sampling was performed at 6-month intervals during a three-year period. First we used conventional chromosomal aberrations analysis. When the aberration frequency for a particular subject reached the background, we measured translocations in the final sample, using fluorescence in situ hybridization. Chromosome painting probes for chromosomes 1, 2, and 4 were used simultaneously. RESULTS: Dicentric and ring chromosomes were eliminated within a year. Translocations persisted and deviated from control values in all examinees. Translocations were detected long after unstable aberrations decreased to the background level. CONCLUSION: Fluorescence in situ hybridization-based translocation detection was a reliable method for monitoring chronic occupational clastogen exposure. Chromosome aberration assay correlated with translocation frequency. Stable chromosomal aberrations reflected cumulative genome damage during job exposure.
AIM: To evaluate chromosome aberration and fluorescent in situ hybridization (FISH) assays as a method to estimate of health risk, we monitored 9 male subjects occupationally exposed to low doses of both ionizing radiation and ultrasound during a period of over 3 years. METHODS: Sampling was performed at 6-month intervals during a three-year period. First we used conventional chromosomal aberrations analysis. When the aberration frequency for a particular subject reached the background, we measured translocations in the final sample, using fluorescence in situ hybridization. Chromosome painting probes for chromosomes 1, 2, and 4 were used simultaneously. RESULTS: Dicentric and ring chromosomes were eliminated within a year. Translocations persisted and deviated from control values in all examinees. Translocations were detected long after unstable aberrations decreased to the background level. CONCLUSION: Fluorescence in situ hybridization-based translocation detection was a reliable method for monitoring chronic occupational clastogen exposure. Chromosome aberration assay correlated with translocation frequency. Stable chromosomal aberrations reflected cumulative genome damage during job exposure.
Authors: H Ashush; L A Rozenszajn; M Blass; M Barda-Saad; D Azimov; J Radnay; D Zipori; U Rosenschein Journal: Cancer Res Date: 2000-02-15 Impact factor: 12.701
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Authors: S Bonassi; L Hagmar; U Strömberg; A H Montagud; H Tinnerberg; A Forni; P Heikkilä; S Wanders; P Wilhardt; I L Hansteen; L E Knudsen; H Norppa Journal: Cancer Res Date: 2000-03-15 Impact factor: 12.701
Authors: Mark P Little; Deukwoo Kwon; Kazataka Doi; Steven L Simon; Dale L Preston; Michele M Doody; Terrence Lee; Jeremy S Miller; Diane M Kampa; Parveen Bhatti; James D Tucker; Martha S Linet; Alice J Sigurdson Journal: Radiat Res Date: 2014-06-16 Impact factor: 2.841