Literature DB >> 175788

Behaviour of tritium-labelled isopenicillin N and 6-aminopenicillanic acid as potential penicillin precursors in an extract of Penicillum chrysogenum.

P A Fawcett, J J Usher, E P Abraham.   

Abstract

1. 3H was incorporated into solvent-soluble penicillin from isopenicillin N and 6-aminopenicillanic acid 3H-labelled in the 2beta-methyl group when the labelled compounds were incubated with a crude extract of Penicillum chrysogenum. 2. With a soluble protein fraction of the extract incorporation from isopenicillin N occurred on addition of phenyl-acetyl-CoA. 3. Labelled benzylpenicillin was isolated after incubation of the crude extract with phenylacetyl-CoA and isopenicillin and the addition of unlabelled benzylpenicillin as a carrier. 4. No incorporation of 3H into solvent-soluble penicillin was detected on incubation of these extracts with penicillin N.

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Year:  1975        PMID： 175788      PMCID： PMC1172423          DOI： 10.1042/bj1510741

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  20 in total

1.  VARIETY OF SUBSTRATES FOR A BACTERIAL BENZYL PENICILLIN-SPLITTING ENZYME.

Authors:  W KAUFMANN; K BAUER
Journal:  Nature       Date:  1964-08-01       Impact factor: 49.962

2.  Aminoadipylpenicillin in penicillin fermentations.

Authors:  M COLE; F R BATCHELOR
Journal:  Nature       Date:  1963       Impact factor: 49.962

3.  Note on suitable solvent systems usable in the liquid scintillation counting of animal tissue.

Authors:  G A BRUNO; J E CHRISTIAN
Journal:  J Am Pharm Assoc Am Pharm Assoc       Date:  1960-08

4.  Synthesis of penicillin: 6-aminopenicillanic acid in penicillin fermentations.

Authors:  F R BATCHELOR; F P DOYLE; J H NAYLER; G N ROLINSON
Journal:  Nature       Date:  1959-01-24       Impact factor: 49.962

5.  Colorimetric detection of penicillins and cephalosporins on paper.

Authors:  R THOMAS
Journal:  Nature       Date:  1961-09-16       Impact factor: 49.962

6.  The structure of a peptide, containing alpha-aminoadipic acid, cystine and valine, present in the mycelium of Penicillium chrysogenum.

Authors:  H R ARNSTEIN; D MORRIS
Journal:  Biochem J       Date:  1960-08       Impact factor: 3.857

7.  A micro biuret method for protein determination; determination of total protein in cerebrospinal fluid.

Authors:  J GOA
Journal:  Scand J Clin Lab Invest       Date:  1953       Impact factor: 1.713

8.  A Comparison of Penicillin-producing Strains of Penicillium notatum-chrysogenum.

Authors:  F B Gailey; J J Stefaniak; B H Olson; M J Johnson
Journal:  J Bacteriol       Date:  1946-07       Impact factor: 3.490

9.  Degradation, structure and some derivatives of cephalosporin N.

Authors:  G G NEWTON; E P ABRAHAM
Journal:  Biochem J       Date:  1954-09       Impact factor: 3.857

10.  Synthesis of tritium-labelled isopenicillin N, penicillin N and 6-aminopenicillanic acid.

Authors:  J J Usher; B Loder; E P Abraham
Journal:  Biochem J       Date:  1975-12       Impact factor: 3.857
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  7 in total

1.  Purification to homogeneity and characterization of acyl coenzyme A:6-aminopenicillanic acid acyltransferase of Penicillium chrysogenum.

Authors:  E Alvarez; J M Cantoral; J L Barredo; B Díez; J F Martín
Journal:  Antimicrob Agents Chemother       Date:  1987-11       Impact factor: 5.191

2.  Structural studies on the reaction of isopenicillin N synthase with the substrate analogue delta-(l-alpha-aminoadipoyl)-l-cysteinyl-d-alpha-aminobutyrate.

Authors:  Alexandra J Long; Ian J Clifton; Peter L Roach; Jack E Baldwin; Christopher J Schofield; Peter J Rutledge
Journal:  Biochem J       Date:  2003-06-15       Impact factor: 3.857

3.  Investigations into the post-translational modification and mechanism of isopenicillin N:acyl-CoA acyltransferase using electrospray mass spectrometry.

Authors:  R T Aplin; J E Baldwin; P L Roach; C V Robinson; C J Schofield
Journal:  Biochem J       Date:  1993-09-01       Impact factor: 3.857

4.  Purification of isopenicillin N synthetase.

Authors:  C P Pang; B Chakravarti; R M Adlington; H H Ting; R L White; G S Jayatilake; J E Baldwin; E P Abraham
Journal:  Biochem J       Date:  1984-09-15       Impact factor: 3.857

5.  Homology modeling of the structure of acyl coA:isopenicillin N-acyltransferase (IAT) from Penicillium chrysogenum. IAT interaction studies with isopenicillin-N, combining molecular dynamics simulations and docking.

Authors:  Liliana Moreno-Vargas; Jose Correa-Basurto; Rachid C Maroun; Francisco J Fernández
Journal:  J Mol Model       Date:  2011-06-22       Impact factor: 1.810

Review 6.  Molecular regulation of beta-lactam biosynthesis in filamentous fungi.

Authors:  A A Brakhage
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

7.  Synthesis of delta-(alpha-aminoadipyl)cysteinylvaline and its role in penicillin biosynthesis.

Authors:  P A Fawcett; J J Usher; J A Huddleston; R C Bleaney; J J Nisbet; E P Abraham
Journal:  Biochem J       Date:  1976-09-01       Impact factor: 3.857
  7 in total

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