Literature DB >> 17535265

A technique for improved focused ion beam milling of cryo-prepared life science specimens.

M F Hayles1, D J Stokes, D Phifer, K C Findlay.   

Abstract

The combination of focused ion beam and scanning electron microscopy with a cryo-preparation/transfer system allows specimens to be milled at low temperatures. However, for biological specimens in particular, the quality of results is strongly dependent on correct preparation of the specimen surface. We demonstrate a method for deposition of a protective, planarizing surface layer onto a cryo-sample, enabling high-quality cross-sectioning using the ion beam and investigation of structures at the nanoscale.

Year:  2007        PMID： 17535265     DOI： 10.1111/j.1365-2818.2007.01775.x

Source DB:  PubMed          Journal:  J Microsc        ISSN： 0022-2720            Impact factor:   1.758


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