Literature DB >> 17512139

Development of a set of plasmid vectors for genetic manipulations of the pathogenic yeast Candida parapsilosis.

Peter Kosa1, Barbora Gavenciakova, Jozef Nosek.   

Abstract

A system for genetic transformation of the yeast Candida parapsilosis, recently developed in our laboratory, opened a venue for investigation of this pathogenic species at the molecular level. In this study we extend the range of available experimental tools by construction of a genomic DNA library suitable for screening and isolation of genes by functional complementation of yeast mutants and a set of replicative plasmid vectors for genetic manipulation of C. parapsilosis cells. The plasmids are based on auxotrophic (CpGAL1, CpURA3, CpMET2, CpLYS4) and dominant (CaIMH3) selection markers. In addition, we constructed plasmid derivatives containing reporter genes yEGFP3 and KlLAC4 coding for enhanced version of the green fluorescent protein and Kluyveromyces lactis beta-galactosidase, respectively. The vectors facilitate propagation and expression of cloned genes in C. parapsilosis cells and allow intracellular localization of gene products and/or monitoring the activity of promoter sequences.

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Year:  2007        PMID： 17512139      PMCID： PMC1994580          DOI： 10.1016/j.gene.2007.04.008

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  42 in total

1.  Development of Streptococcus thermophilus lacZ as a reporter gene for Candida albicans.

Authors:  M Andrew Uhl; Alexander D Johnson
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2.  Secreted aspartic proteases of Candida albicans, Candida tropicalis, Candida parapsilosis and Candida lusitaniae. Inhibition with peptidomimetic inhibitors.

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Journal:  Eur J Biochem       Date:  2001-05

3.  New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites.

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4.  Vectors for gene expression and amplification in the yeast Yarrowia lipolytica.

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Journal:  Yeast       Date:  2001-01-30       Impact factor: 3.239

5.  Candidemia in a tertiary care hospital: epidemiology and factors influencing mortality.

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6.  Genetic manipulation of the pathogenic yeast Candida parapsilosis.

Authors:  Jozef Nosek; Lubica Adamíková; Júlia Zemanová; Lubomír Tomáska; Rachel Zufferey; Choukri Ben Mamoun
Journal:  Curr Genet       Date:  2002-09-20       Impact factor: 3.886

7.  Candidaemia in special care nurseries: comparison of albicans and parapsilosis infection.

Authors:  Y C Huang; T Y Lin; R I Lien; Y H Chou; C Y Kuo; P H Yang; W S Hsieh
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8.  A molecular genetic system for the pathogenic yeast Candida dubliniensis.

Authors:  P Staib; S Michel; G Köhler; J Morschhäuser
Journal:  Gene       Date:  2000-01-25       Impact factor: 3.688

9.  Mitochondrial telomeres as molecular markers for identification of the opportunistic yeast pathogen Candida parapsilosis.

Authors:  Jozef Nosek; L'ubomír Tomáska; Adriana Rycovská; Hiroshi Fukuhara
Journal:  J Clin Microbiol       Date:  2002-04       Impact factor: 5.948

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Authors:  L Tomaska; J Nosek; A M Makhov; A Pastorakova; J D Griffith
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  8 in total

Review 1.  Candida parapsilosis: from Genes to the Bedside.

Authors:  Renáta Tóth; Jozef Nosek; Héctor M Mora-Montes; Toni Gabaldon; Joseph M Bliss; Joshua D Nosanchuk; Siobhán A Turner; Geraldine Butler; Csaba Vágvölgyi; Attila Gácser
Journal:  Clin Microbiol Rev       Date:  2019-02-27       Impact factor: 26.132

Review 2.  Biology and genetics of the pathogenic yeast Candida parapsilosis.

Authors:  Jozef Nosek; Zuzana Holesova; Peter Kosa; Attila Gacser; Lubomir Tomaska
Journal:  Curr Genet       Date:  2009-08-07       Impact factor: 3.886

3.  Candida guilliermondii: biotechnological applications, perspectives for biological control, emerging clinical importance and recent advances in genetics.

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Journal:  Curr Genet       Date:  2013-04-25       Impact factor: 3.886

4.  Exploiting Issatchenkia orientalis SD108 for succinic acid production.

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5.  Mitochondrial Carriers Link the Catabolism of Hydroxyaromatic Compounds to the Central Metabolism in Candida parapsilosis.

Authors:  Igor Zeman; Martina Neboháčová; Gabriela Gérecová; Kornélia Katonová; Eva Jánošíková; Michaela Jakúbková; Ivana Centárová; Ivana Dunčková; L'ubomír Tomáška; Leszek P Pryszcz; Toni Gabaldón; Jozef Nosek
Journal:  G3 (Bethesda)       Date:  2016-12-07       Impact factor: 3.154

6.  Eukaryotic transporters for hydroxyderivatives of benzoic acid.

Authors:  Andrea Cillingová; Igor Zeman; Renáta Tóth; Martina Neboháčová; Ivana Dunčková; Mária Hölcová; Michaela Jakúbková; Gabriela Gérecová; Leszek P Pryszcz; Ľubomír Tomáška; Toni Gabaldón; Attila Gácser; Jozef Nosek
Journal:  Sci Rep       Date:  2017-08-21       Impact factor: 4.379

7.  Transcriptome and proteome profiling reveals complex adaptations of Candida parapsilosis cells assimilating hydroxyaromatic carbon sources.

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Journal:  PLoS Genet       Date:  2022-03-07       Impact factor: 5.917

8.  The structure and DNA-binding properties of Mgm101 from a yeast with a linear mitochondrial genome.

Authors:  Vladimír Pevala; Dominika Truban; Jacob A Bauer; Július Košťan; Nina Kunová; Jana Bellová; Marlene Brandstetter; Victoria Marini; Lumír Krejčí; Ľubomír Tomáška; Jozef Nosek; Eva Kutejová
Journal:  Nucleic Acids Res       Date:  2016-01-06       Impact factor: 16.971
  8 in total

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