Forty mouse strain survey of body composition.

We measured body weight and composition of approximately 10 male and approximately 10 female mice from 40 inbred strains. Body composition was assessed in approximately 16-wk old mice that had been individually housed and fed a high-carbohydrate, low-fat diet (AIN-76A) for the previous 8 wk. Carcass lean and fat weights were assessed using a PIXIMus II DEXA and confirmed by fat extraction assay. There was a nearly continuous range of body weights, from a strain mean+/-SE of 11.4+/-0.2 g (MSM/MsJ) to 39.3+/-1.8 g (NON/LtJ). The percentage of body weight that was fat (%Fat) ranged from 16+/-4% (C58/J) to 39+/-2% (NON/LtJ). In general, heavier strains had a higher %Fat (r=0.57) but several light strains were also quite fat (e.g., SPRET/EiJ, body weight=15.7+/-0.6 g, %Fat=26+/-1%). Males were significantly heavier than females in 26 strains and significantly fatter than females in 9 strains; only the KK/H1J strain had fatter females than males. Some of the fattest strains are infrequently used in obesity experiments, for example the JF1/Ms and CBA/J strains. These data illustrate the diversity of body weight and composition in inbred mice. They will serve as a reference standard and assist in the selection of strains for future work.