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Literature DB >> 17483260

Self-formed adaptor PCR: a simple and efficient method for chromosome walking.

Shiming Wang¹, Jian He, Zhongli Cui, Shunpeng Li.

Abstract

We developed a self-formed adaptor PCR (termed SEFA PCR) which can be used for chromosome walking. Most of the amplified flanking sequences were longer than 2.0 kb, and some were as long as 6.0 kb. SEFA PCR is simple and efficient and should have broad applications in the isolation of unknown sequences in complex genomes.

Mesh：

Substances：
DNA Primers

Year: 2007 PMID： 17483260 PMCID： PMC1951051 DOI： 10.1128/AEM.02973-06

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

19 in total

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