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Literature DB >> 2106670

Cloning regions of the Drosophila genome by microdissection of polytene chromosome DNA and PCR with nonspecific primer.

C S Wesley¹, M Ben, M Kreitman, N Hagag, W F Eanes.

Abstract

A simple and rapid procedure to isolate clones carrying sequences from a specific region of the polytene chromosome of Drosophila is demonstrated. The procedure involves microdissection of the region of interest, amplification of the DNA by PCR using a primer designed to prime the synthesis nonspecifically, labeling of the amplified DNA using the random primer method, and screening of a standard library with the probe to identify and isolate clones carrying sequences homologous to the dissected region. This procedure has the potential to replace the difficult procedure of microcloning, as well as facilitate chromosome walking.

Entities: Species

Mesh：

Substances：
DNA Probes
Biotin
DNA

Year: 1990 PMID： 2106670 PMCID： PMC333467 DOI： 10.1093/nar/18.3.599

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

6 in total

1. Nucleic acid hybridization to the DNA of cytological preparations.

Authors: M L Pardue; J G Gall
Journal: Methods Cell Biol Date: 1975 Impact factor: 1.441

2. A test for the role of natural selection in the stabilization of transposable element copy number in a population of Drosophila melanogaster.

Authors: E Montgomery; B Charlesworth; C H Langley
Journal: Genet Res Date: 1987-02 Impact factor: 1.588

3. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors: R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal: Science Date: 1988-01-29 Impact factor: 47.728

4. Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification.

Authors: H J Lüdecke; G Senger; U Claussen; B Horsthemke
Journal: Nature Date: 1989-03-23 Impact factor: 49.962

5. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors: A P Feinberg; B Vogelstein
Journal: Anal Biochem Date: 1983-07-01 Impact factor: 3.365

6. Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.

Authors: P R Langer; A A Waldrop; D C Ward
Journal: Proc Natl Acad Sci U S A Date: 1981-11 Impact factor: 11.205

6 in total

11 in total

1. Atomic force microscopy and scanning near-field optical microscopy studies on the characterization of human metaphase chromosomes.

Authors: M Oberringer; A Englisch; B Heinz; H Gao; T Martin; U Hartmann
Journal: Eur Biophys J Date: 2003-07-09 Impact factor: 1.733

Cloning regions of the Drosophila genome by microdissection of polytene chromosome DNA and PCR with nonspecific primer.

1. Nucleic acid hybridization to the DNA of cytological preparations.

2. A test for the role of natural selection in the stabilization of transposable element copy number in a population of Drosophila melanogaster.

3. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

4. Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification.

5. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

6. Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.

1. Atomic force microscopy and scanning near-field optical microscopy studies on the characterization of human metaphase chromosomes.

2. Isolation of region-specific cosmids from chromosome 5 by hybridization with microdissection clones.

3. Self-formed adaptor PCR: a simple and efficient method for chromosome walking.

4. Imaging and nanodissection of individual supercoiled plasmids by atomic force microscopy.

5. Vector-hexamer PCR isolation of all insert ends from a YAC contig of the mouse Igh locus.

6. Chromosome microdissection and microcloning.

7. Libraries for each human chromosome, constructed from sorter-enriched chromosomes by using linker-adaptor PCR.

8. Rapid sequential walking from termini of cosmid, P1 and YAC inserts.

9. Fingerprinting genomes using PCR with arbitrary primers.

10. Isolation and analysis of the breakpoint sequences of chromosome inversion In(3L)Payne in Drosophila melanogaster.