| Literature DB >> 174724 |
R J Delap, D Zouzias, M G Rush.
Abstract
A method is described for the preparation of 125I-labelled SV40 DNA. Using this method, SV40 DNA can be routinely labelled to 15 - 10(6) dpm per mug; much higher specific activities are easily obtained by minor modifications of the method. Once incorporated, the radioactive label dissociates from DNA exceedingly slowly at 4 degrees C or at 68 degrees C. Iodinated SV40 DNA is shown to be useful in the quantitation of viral nucleic acid sequences in SV40-transformed 3T3 cells by DNA - DNA reassociation kinetics.Entities:
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Year: 1976 PMID： 174724 DOI： 10.1016/0005-2787(76)90288-4
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002