BACKGROUND: Oxidative stress is a complicating factor in chronic renal failure, especially in hemodialysis (HD) patients. Also, aluminum intoxication may occur during hemodialysis treatment. Aluminum has been shown to inhibit the sulfhydryl-containing enzyme delta-aminolevulinate dehydratase (ALA-D). Thus, the involvement of -SH oxidation in ALA-D inhibition and its relationship with serum Al levels and lipid peroxidation in HD patients were evaluated. METHODS: Blood ALA-D activity, plasma thiobarbituric acid reactive substances (TBARS), and serum aluminum levels were measured in HD patients (n=37) and healthy controls (n=20). RESULTS: TBARS and Al levels were higher in HD patients than in controls (p<0.01), while ALA-D activity was lower (p<0.05). The sulfhydryl-reducing agent dithiothreitol (DTT) reactivated ALA-D of HD patients, but activity was still lower than that of controls. ALA-D activity was negatively correlated with TBARS (r=-0.63, p<0.01) and aluminum levels (r=-0.31, p<0.05). CONCLUSIONS: Reduced ALA-D activity in HD patients was found to be related to the oxidation of -SH groups essential for enzyme activity. Our results suggest that increased oxidative stress may have contributed to enzyme inhibition in HD patients.
BACKGROUND: Oxidative stress is a complicating factor in chronic renal failure, especially in hemodialysis (HD) patients. Also, aluminum intoxication may occur during hemodialysis treatment. Aluminum has been shown to inhibit the sulfhydryl-containing enzyme delta-aminolevulinate dehydratase (ALA-D). Thus, the involvement of -SH oxidation in ALA-D inhibition and its relationship with serum Al levels and lipid peroxidation in HDpatients were evaluated. METHODS: Blood ALA-D activity, plasma thiobarbituric acid reactive substances (TBARS), and serum aluminum levels were measured in HDpatients (n=37) and healthy controls (n=20). RESULTS:TBARS and Al levels were higher in HDpatients than in controls (p<0.01), while ALA-D activity was lower (p<0.05). The sulfhydryl-reducing agent dithiothreitol (DTT) reactivated ALA-D of HDpatients, but activity was still lower than that of controls. ALA-D activity was negatively correlated with TBARS (r=-0.63, p<0.01) and aluminum levels (r=-0.31, p<0.05). CONCLUSIONS: Reduced ALA-D activity in HDpatients was found to be related to the oxidation of -SH groups essential for enzyme activity. Our results suggest that increased oxidative stress may have contributed to enzyme inhibition in HDpatients.
Authors: Luiz Carlos Cichota; Rafael Noal Moresco; Marta Maria Medeiros Frescura Duarte; José Edson Paz da Silva Journal: J Clin Lab Anal Date: 2008 Impact factor: 2.352
Authors: Thissiane L Gonçalves; Dalila M Benvegnú; Gabriela Bonfanti; Andressa V Frediani; João Batista T Rocha Journal: BMC Cancer Date: 2009-05-08 Impact factor: 4.430
Authors: Sabrina Nunes do Nascimento; Mariele Feiffer Charão; Angela Maria Moro; Miguel Roehrs; Clovis Paniz; Marília Baierle; Natália Brucker; Adriana Gioda; Fernando Barbosa; Denise Bohrer; Daiana Silva Ávila; Solange Cristina Garcia Journal: Int J Environ Res Public Health Date: 2014-10-17 Impact factor: 3.390
Authors: Marília Baierle; Mariele F Charão; Gabriela Göethel; Anelise Barth; Rafael Fracasso; Guilherme Bubols; Elisa Sauer; Sarah C Campanharo; Rafael C C Rocha; Tatiana D Saint'Pierre; Suelen Bordignon; Murilo Zibetti; Clarissa M Trentini; Daiana S Avila; Adriana Gioda; Solange C Garcia Journal: Int J Environ Res Public Health Date: 2014-10-17 Impact factor: 3.390