BACKGROUND: Oxidative stress (OS) is reportedly enhanced in patients receiving regular hemodialysis (HD). However, the in vivo redox state of HD patients, particularly after HD sessions, remains unclear. This study aimed to clarify the influence of HD on OS using the albumin redox state as a marker. METHOD: Blood samples of 8 regular HD patients were obtained during the course of study. The redox state of albumin was determined using high-performance liquid chromatography. RESULTS: The mean fraction of reversibly oxidized albumin [f(HNA-1)] declined significantly over the course of the session and reached a minimum 4 h after the session had ended (pre-HD, 36.16 +/- 7.50%; 4 h after HD, 25.71 +/- 6.41%), then gradually rose to predialytic levels. The proportion of irreversibly oxidized albumin did not change significantly over time. Positive correlations were demonstrated between f(HNA-1) and uremic small solutes in each case. CONCLUSION: Accumulation of dialyzable uremic solutes may contribute to OS in HD patients. Copyright 2007 S. Karger AG, Basel.
BACKGROUND: Oxidative stress (OS) is reportedly enhanced in patients receiving regular hemodialysis (HD). However, the in vivo redox state of HDpatients, particularly after HD sessions, remains unclear. This study aimed to clarify the influence of HD on OS using the albumin redox state as a marker. METHOD: Blood samples of 8 regular HDpatients were obtained during the course of study. The redox state of albumin was determined using high-performance liquid chromatography. RESULTS: The mean fraction of reversibly oxidized albumin [f(HNA-1)] declined significantly over the course of the session and reached a minimum 4 h after the session had ended (pre-HD, 36.16 +/- 7.50%; 4 h after HD, 25.71 +/- 6.41%), then gradually rose to predialytic levels. The proportion of irreversibly oxidized albumin did not change significantly over time. Positive correlations were demonstrated between f(HNA-1) and uremic small solutes in each case. CONCLUSION: Accumulation of dialyzable uremic solutes may contribute to OS in HDpatients. Copyright 2007 S. Karger AG, Basel.
Authors: Lucía Turell; Horacio Botti; Lucía Bonilla; María José Torres; Francisco Schopfer; Bruce A Freeman; Larissa Armas; Alejandro Ricciardi; Beatriz Alvarez; Rafael Radi Journal: J Chromatogr B Analyt Technol Biomed Life Sci Date: 2013-11-20 Impact factor: 3.205
Authors: Joshua W Jeffs; Nilojan Jehanathan; Stephanie M F Thibert; Shadi Ferdosi; Linda Pham; Zachary T Wilson; Christian Breburda; Chad R Borges Journal: Mol Cell Proteomics Date: 2019-07-19 Impact factor: 5.911