PURPOSE: To clarify the redox states of the aqueous humor and serum in senile cataract patients. METHODS: We analyzed the reduced and oxidized albumin fractions in 58 patients. The patients had no detectable evidence of other eye or systemic diseases and were divided into two age groups (53-75 and 76-96 years). Analysis was performed with a high-performance liquid chromatographic system with a fluorescence detector. RESULTS: Extremely small amounts of reduced albumin (3.8% and 3.6% in the younger and older groups, respectively) and markedly large amounts of oxidized albumin (96.2% and 96.4%, respectively) were found in the aqueous humor. Moreover, a higher percentage of oxidized albumin, particularly a highly oxidized irreversible form of albumin, was found in the aqueous humor (12.3% and 12.0%) than in the serum (2.4% and 3.0%). CONCLUSIONS: In senile cataract patients with no complications, the redox state of albumin in the aqueous humor differs considerably, both quantitatively and qualitatively, from that in the serum.
PURPOSE: To clarify the redox states of the aqueous humor and serum in senile cataractpatients. METHODS: We analyzed the reduced and oxidized albumin fractions in 58 patients. The patients had no detectable evidence of other eye or systemic diseases and were divided into two age groups (53-75 and 76-96 years). Analysis was performed with a high-performance liquid chromatographic system with a fluorescence detector. RESULTS: Extremely small amounts of reduced albumin (3.8% and 3.6% in the younger and older groups, respectively) and markedly large amounts of oxidized albumin (96.2% and 96.4%, respectively) were found in the aqueous humor. Moreover, a higher percentage of oxidized albumin, particularly a highly oxidized irreversible form of albumin, was found in the aqueous humor (12.3% and 12.0%) than in the serum (2.4% and 3.0%). CONCLUSIONS: In senile cataractpatients with no complications, the redox state of albumin in the aqueous humor differs considerably, both quantitatively and qualitatively, from that in the serum.
Authors: Sebastián Carballal; Rafael Radi; Marion C Kirk; Stephen Barnes; Bruce A Freeman; Beatriz Alvarez Journal: Biochemistry Date: 2003-08-26 Impact factor: 3.162