Literature DB >> 17448143

Selection strategies for improved biocatalysts.

Ykelien L Boersma1, Melloney J Dröge, Wim J Quax.   

Abstract

Enzymes have become an attractive alternative to conventional catalysts in numerous industrial processes. However, their properties do not always meet the criteria of the application of interest. Directed evolution is a powerful tool for adopting the characteristics of an enzyme. However, selection of the evolved variants is a critical step, and therefore new strategies to enable selection of the desired enzymatic activity have been developed. This review focuses on these novel strategies for selecting enzymes from large libraries, in particular those that are used in the synthesis of pharmaceutical intermediates and pharmaceuticals.

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Year:  2007        PMID： 17448143     DOI： 10.1111/j.1742-4658.2007.05782.x

Source DB:  PubMed          Journal:  FEBS J        ISSN： 1742-464X            Impact factor:   5.542


  11 in total

1.  Highly L and D enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection method.

Authors:  Eugene Antipov; Art E Cho; K Dane Wittrup; Alexander M Klibanov
Journal:  Proc Natl Acad Sci U S A       Date:  2008-11-12       Impact factor: 11.205

2.  Rescuing auxotrophic microorganisms with nonenzymatic chemistry.

Authors:  Yunmi Lee; Afoma Umeano; Emily P Balskus
Journal:  Angew Chem Int Ed Engl       Date:  2013-09-20       Impact factor: 15.336

3.  Assessing directed evolution methods for the generation of biosynthetic enzymes with potential in drug biosynthesis.

Authors:  David P Nannemann; William R Birmingham; Robert A Scism; Brian O Bachmann
Journal:  Future Med Chem       Date:  2011-05       Impact factor: 3.808

Review 4.  Learning Strategies in Protein Directed Evolution.

Authors:  Xavier F Cadet; Jean Christophe Gelly; Aster van Noord; Frédéric Cadet; Carlos G Acevedo-Rocha
Journal:  Methods Mol Biol       Date:  2022

5.  A growth- and bioluminescence-based bioreporter for the in vivo detection of novel biocatalysts.

Authors:  Teunke van Rossum; Aleksandra Muras; Marco J J Baur; Sjoerd C A Creutzburg; John van der Oost; Servé W M Kengen
Journal:  Microb Biotechnol       Date:  2017-04-10       Impact factor: 5.813

Review 6.  Engineering of L-amino acid deaminases for the production of α-keto acids from L-amino acids.

Authors:  Project Nshimiyimana; Long Liu; Guocheng Du
Journal:  Bioengineered       Date:  2019-12       Impact factor: 3.269

Review 7.  High-throughput droplet-based microfluidics for directed evolution of enzymes.

Authors:  Flora W Y Chiu; Stavros Stavrakis
Journal:  Electrophoresis       Date:  2019-08-29       Impact factor: 3.535

8.  Enhancement of protein thermostability by three consecutive mutations using loop-walking method and machine learning.

Authors:  Kazunori Yoshida; Shun Kawai; Masaya Fujitani; Satoshi Koikeda; Ryuji Kato; Tadashi Ema
Journal:  Sci Rep       Date:  2021-06-04       Impact factor: 4.379

Review 9.  High-Throughput Screening in Protein Engineering: Recent Advances and Future Perspectives.

Authors:  Magdalena Wójcik; Aline Telzerow; Wim J Quax; Ykelien L Boersma
Journal:  Int J Mol Sci       Date:  2015-10-20       Impact factor: 5.923

Review 10.  Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently.

Authors:  Andrew Currin; Neil Swainston; Philip J Day; Douglas B Kell
Journal:  Chem Soc Rev       Date:  2015-03-07       Impact factor: 54.564
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