| Literature DB >> 17435763 |
Sangjin Kim1, Paul C Blainey, Charles M Schroeder, X Sunney Xie.
Abstract
We report a single-molecule assay for nucleic-acid enzymes on flow-stretched DNA templates. To facilitate the detection of slow or intermittent enzymatic activities, we developed the assay with 15-nm spatial resolution at a frame rate of 1 Hz and approximately 10 nm mechanical stability over the timescale of hours. With multiplexed data collection, we applied the assay to phi29 DNA polymerase, HIV-1 reverse transcriptase, lambda exonuclease and Escherichia coli RNA polymerase.Entities:
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Year: 2007 PMID： 17435763 DOI： 10.1038/nmeth1037
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547