| Literature DB >> 17382934 |
Abstract
An NADH-dependent trans-2-enoyl-CoA reductase (EC1.1.1.36) from the Gram negative spirochete Treponema denticola was identified, expressed and biochemically characterized. The recombinant protein is a monomeric enzyme with a molecular mass of 44 kDa with a specific activity of 43+/-4.8 U/mg (micromol mg(-1)min(-1)) and K(m) value of 2.7 microM for crotonoyl-CoA. This NADH-dependent trans-2-enoyl-CoA reductase represents the first enzymatically characterized member of a prokaryotic protein family involved in a fatty acid synthesis pathway that is distinct from the familiar fatty acid synthase system.Entities:
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Year: 2007 PMID： 17382934 DOI： 10.1016/j.febslet.2007.03.013
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124