| Literature DB >> 17381285 |
C P Hunter1, W M Winston, C Molodowitch, E H Feinberg, J Shih, M Sutherlin, A J Wright, M C Fitzgerald.
Abstract
RNA interference (RNAi) in Caenorhabditis elegans induced by ingestion or injection of double-stranded RNA (dsRNA) spreads throughout the organism and is even transmitted to the progeny. We have identified two proteins required for spreading of RNAi, SID-1 and SID-2, whose structure, subcellular localization, and expression pattern have been informative for how dsRNA can be transported into and between cells. SID-1 is a transmembrane protein that functions as a pore or channel that transports dsRNA into and out of cells. Proteins homologous to SID-1 are present in a wide range of invertebrate and vertebrate animals but are absent from plants. SID-2 is a small transmembrane protein that is expressed in the gut and localizes strongly to the luminal membrane where it appears to act as a receptor for uptake of dsRNA from the environment. Characterization of SID-2 activity in a variety of Caenorhabditis nematodes indicates that C. elegans SID-2 may have a novel activity.Entities:
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Year: 2006 PMID： 17381285 DOI： 10.1101/sqb.2006.71.060
Source DB: PubMed Journal: Cold Spring Harb Symp Quant Biol ISSN： 0091-7451