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Literature DB >> 10639176

Constraining the subunit order of rod cyclic nucleotide-gated channels reveals a diagonal arrangement of like subunits.

Abstract

Retinal rod cyclic nucleotide-gated channels are composed of alpha and beta subunits. We have explored possible subunit arrangements by expressing tandemly linked dimers of both subunits and examining their responses to three different modulating agents. Channels formed from either alpha-beta or beta-alpha heterodimers had functional properties similar to those formed from coexpressed alpha and beta monomers and to native channels. These results point to an alpha-beta-alpha-beta arrangement. To ensure that heterodimers had not flipped around, we coexpressed alpha-alpha dimers with an excess of either beta monomers or beta-beta dimers. Our data indicate that heteromultimers do not form efficiently in an alpha-alpha-beta-beta arrangement. Thus, we propose that native rod cyclic nucleotide-gated channels are arranged with like subunits diagonally opposed: alpha-beta-alpha-beta.

Entities: Chemical

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Year: 2000 PMID： 10639176 PMCID： PMC15427 DOI： 10.1073/pnas.97.2.895

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

48 in total

1. Single cyclic nucleotide-gated channels locked in different ligand-bound states.

Authors: M L Ruiz; J W Karpen
Journal: Nature Date: 1997-09-25 Impact factor: 49.962

2. Stoichiometry and arrangement of heteromeric olfactory cyclic nucleotide-gated ion channels.

Authors: M S Shapiro; W N Zagotta
Journal: Proc Natl Acad Sci U S A Date: 1998-11-24 Impact factor: 11.205

Review 3. Molecular and pharmacological analysis of cyclic nucleotide-gated channel function in the central nervous system.

Authors: J Y Wei; D S Roy; L Leconte; C J Barnstable
Journal: Prog Neurobiol Date: 1998-10 Impact factor: 11.685

Review 4. Photoreceptor membrane proteins, phototransduction, and retinal degenerative diseases. The Friedenwald Lecture.

Authors: R S Molday
Journal: Invest Ophthalmol Vis Sci Date: 1998-12 Impact factor: 4.799

5. The structure of the potassium channel: molecular basis of K+ conduction and selectivity.

Authors: D A Doyle; J Morais Cabral; R A Pfuetzner; A Kuo; J M Gulbis; S L Cohen; B T Chait; R MacKinnon
Journal: Science Date: 1998-04-03 Impact factor: 47.728

6. Subunit interactions in the activation of cyclic nucleotide-gated ion channels.

Authors: M D Varnum; W N Zagotta
Journal: Biophys J Date: 1996-06 Impact factor: 4.033

7. A cyclic nucleotide-gated conductance in olfactory receptor cilia.

Authors: T Nakamura; G H Gold
Journal: Nature Date: 1987 Jan 29-Feb 4 Impact factor: 49.962

8. An isoform of the rod photoreceptor cyclic nucleotide-gated channel beta subunit expressed in olfactory neurons.

Authors: A Sautter; X Zong; F Hofmann; M Biel
Journal: Proc Natl Acad Sci U S A Date: 1998-04-14 Impact factor: 11.205

9. Primary structure and expression of the human beta-subunit and related proteins of the rod photoreceptor cGMP-gated channel.

Authors: C A Colville; R S Molday
Journal: J Biol Chem Date: 1996-12-20 Impact factor: 5.157

10. Intermediate conductances during deactivation of heteromultimeric Shaker potassium channels.

Authors: J Zheng; F J Sigworth
Journal: J Gen Physiol Date: 1998-10 Impact factor: 4.086

13 in total

1. Direct association of ligand-binding and pore domains in homo- and heterotetrameric inositol 1,4,5-trisphosphate receptors.

Authors: D Boehning; S K Joseph
Journal: EMBO J Date: 2000-10-16 Impact factor: 11.598

Constraining the subunit order of rod cyclic nucleotide-gated channels reveals a diagonal arrangement of like subunits.

1. Single cyclic nucleotide-gated channels locked in different ligand-bound states.

2. Stoichiometry and arrangement of heteromeric olfactory cyclic nucleotide-gated ion channels.

Review 3. Molecular and pharmacological analysis of cyclic nucleotide-gated channel function in the central nervous system.

Review 4. Photoreceptor membrane proteins, phototransduction, and retinal degenerative diseases. The Friedenwald Lecture.

5. The structure of the potassium channel: molecular basis of K+ conduction and selectivity.

6. Subunit interactions in the activation of cyclic nucleotide-gated ion channels.

7. A cyclic nucleotide-gated conductance in olfactory receptor cilia.

8. An isoform of the rod photoreceptor cyclic nucleotide-gated channel beta subunit expressed in olfactory neurons.

9. Primary structure and expression of the human beta-subunit and related proteins of the rod photoreceptor cGMP-gated channel.

10. Intermediate conductances during deactivation of heteromultimeric Shaker potassium channels.

1. Direct association of ligand-binding and pore domains in homo- and heterotetrameric inositol 1,4,5-trisphosphate receptors.

2. Mechanism of calcium/calmodulin inhibition of rod cyclic nucleotide-gated channels.

3. Selective heteromeric assembly of cyclic nucleotide-gated channels.

4. Fraction of the dark current carried by Ca(2+) through cGMP-gated ion channels of intact rod and cone photoreceptors.

5. Subunit counting in membrane-bound proteins.

6. Molecular architecture of a retinal cGMP-gated channel: the arrangement of the cytoplasmic domains.

7. The heteromeric cyclic nucleotide-gated channel adopts a 3A:1B stoichiometry.

8. Gating of heteromeric retinal rod channels by cyclic AMP: role of the C-terminal and pore domains.

9. Movement of the C-helix during the gating of cyclic nucleotide-gated channels.

10. Locking CNGA1 channels in the open and closed state.