Literature DB >> 17356761

Quantitative detection of protein expression in single cells using droplet microfluidics.

A Huebner1, M Srisa-Art, D Holt, C Abell, F Hollfelder, A J deMello, J B Edel.   

Abstract

We demonstrate that single cells can be controllably compartmentalized within aqueous microdroplets; using such an approach we perform high-throughput screening by detecting the expression of a fluorescent protein in individual cells with simultaneous measurement of droplet size and cell occupancy.

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Year:  2007        PMID： 17356761     DOI： 10.1039/b618570c

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  80 in total

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2.  High throughput single-cell and multiple-cell micro-encapsulation.

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3.  High-throughput injection with microfluidics using picoinjectors.

Authors:  Adam R Abate; Tony Hung; Pascaline Mary; Jeremy J Agresti; David A Weitz
Journal:  Proc Natl Acad Sci U S A       Date:  2010-10-20       Impact factor: 11.205

Review 4.  Microfluidic stochastic confinement enhances analysis of rare cells by isolating cells and creating high density environments for control of diffusible signals.

Authors:  Meghan E Vincent; Weishan Liu; Elizabeth B Haney; Rustem F Ismagilov
Journal:  Chem Soc Rev       Date:  2010-01-12       Impact factor: 54.564

Review 5.  Opportunities for microfluidic technologies in synthetic biology.

Authors:  Shelly Gulati; Vincent Rouilly; Xize Niu; James Chappell; Richard I Kitney; Joshua B Edel; Paul S Freemont; Andrew J deMello
Journal:  J R Soc Interface       Date:  2009-05-27       Impact factor: 4.118

6.  Lab-on-a-chip technologies for proteomic analysis from isolated cells.

Authors:  H Sedgwick; F Caron; P B Monaghan; W Kolch; J M Cooper
Journal:  J R Soc Interface       Date:  2008-10-06       Impact factor: 4.118

7.  PCR-activated cell sorting as a general, cultivation-free method for high-throughput identification and enrichment of virus hosts.

Authors:  Shaun W Lim; Shea T Lance; Kenneth M Stedman; Adam R Abate
Journal:  J Virol Methods       Date:  2016-12-29       Impact factor: 2.014

8.  Self-digitization of samples into a high-density microfluidic bottom-well array.

Authors:  Thomas Schneider; Gloria S Yen; Alison M Thompson; Daniel R Burnham; Daniel T Chiu
Journal:  Anal Chem       Date:  2013-10-07       Impact factor: 6.986

9.  Isolation, incubation, and parallel functional testing and identification by FISH of rare microbial single-copy cells from multi-species mixtures using the combination of chemistrode and stochastic confinement.

Authors:  Weishan Liu; Hyun Jung Kim; Elena M Lucchetta; Wenbin Du; Rustem F Ismagilov
Journal:  Lab Chip       Date:  2009-05-14       Impact factor: 6.799

10.  Droplet microfluidics for amplification-free genetic detection of single cells.

Authors:  Tushar D Rane; Helena C Zec; Chris Puleo; Abraham P Lee; Tza-Huei Wang
Journal:  Lab Chip       Date:  2012-07-30       Impact factor: 6.799
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